Generation of Large Fragment Knock-In Mouse Models by Microinjecting into 2-Cell Stage Embryos.

TitleGeneration of Large Fragment Knock-In Mouse Models by Microinjecting into 2-Cell Stage Embryos.
Publication TypeJournal Article
Year of Publication2020
AuthorsGu, B, Gertsenstein, M, Posfai, E
JournalMethods Mol Biol
Volume2066
Pagination89-100
Date Published2020
ISSN1940-6029
Abstract

Large fragment knock-in mouse models such as reporters and conditional mutant mice are important models for biological research. Here we describe 2-cell (2C)-homologous recombination (HR)-CRISPR, a highly efficient method to generate large fragment knock-in mouse models by CRISPR-based genome engineering. Using this method, knock-in founders can be generated routinely in a time frame of about two months with high germline transmission efficiency. 2C-HR-CRISPR will significantly promote the advancement of basic and translational research using genetic mouse models.

DOI10.1007/978-1-4939-9837-1_7
Alternate JournalMethods Mol. Biol.
PubMed ID31512209