Title | Generation of Large Fragment Knock-In Mouse Models by Microinjecting into 2-Cell Stage Embryos. |
Publication Type | Journal Article |
Year of Publication | 2020 |
Authors | Gu, B, Gertsenstein, M, Posfai, E |
Journal | Methods Mol Biol |
Volume | 2066 |
Pagination | 89-100 |
Date Published | 2020 |
ISSN | 1940-6029 |
Keywords | Animals, CRISPR-Cas Systems, Embryo, Mammalian, Embryonic Development, Gene Editing, Gene Knock-In Techniques, Genome, Homologous Recombination, Mice, Microinjections |
Abstract | <p>Large fragment knock-in mouse models such as reporters and conditional mutant mice are important models for biological research. Here we describe 2-cell (2C)-homologous recombination (HR)-CRISPR, a highly efficient method to generate large fragment knock-in mouse models by CRISPR-based genome engineering. Using this method, knock-in founders can be generated routinely in a time frame of about two months with high germline transmission efficiency. 2C-HR-CRISPR will significantly promote the advancement of basic and translational research using genetic mouse models.</p> |
DOI | 10.1007/978-1-4939-9837-1_7 |
Alternate Journal | Methods Mol Biol |
PubMed ID | 31512209 |
Grant List | FDN-143334 / / CIHR / Canada |