|Title||Generation of Large Fragment Knock-In Mouse Models by Microinjecting into 2-Cell Stage Embryos.|
|Publication Type||Journal Article|
|Year of Publication||2020|
|Authors||Gu, B, Gertsenstein, M, Posfai, E|
|Journal||Methods Mol Biol|
Large fragment knock-in mouse models such as reporters and conditional mutant mice are important models for biological research. Here we describe 2-cell (2C)-homologous recombination (HR)-CRISPR, a highly efficient method to generate large fragment knock-in mouse models by CRISPR-based genome engineering. Using this method, knock-in founders can be generated routinely in a time frame of about two months with high germline transmission efficiency. 2C-HR-CRISPR will significantly promote the advancement of basic and translational research using genetic mouse models.
|Alternate Journal||Methods Mol. Biol.|