Gdf3 is required for robust Nodal signaling during germ layer formation and left-right patterning.

TitleGdf3 is required for robust Nodal signaling during germ layer formation and left-right patterning.
Publication TypeJournal Article
Year of Publication2017
AuthorsPelliccia, JL, Jindal, GA, Burdine, RD
JournalElife
Volume6
Date Published2017 11 15
ISSN2050-084X
KeywordsAnimals, Body Patterning, Germ Layers, Nodal Protein, Signal Transduction, Transforming Growth Factor beta, Zebrafish, Zebrafish Proteins
Abstract

<p>Vertebrate embryonic patterning depends on signaling from Nodal, a TGFβ superfamily member. There are three Nodal orthologs in zebrafish; directs left-right asymmetries, while and function earlier to pattern mesendoderm. TGFβ member Vg1 is implicated in mesoderm formation but the role of the zebrafish ortholog, Growth differentiation factor 3 (Gdf3), has not been fully explored. We show that zygotic expression of is dispensable for embryonic development, while maternally deposited is required for mesendoderm formation and dorsal-ventral patterning. We further show that Gdf3 can affect left-right patterning at multiple stages, including proper development of regional cell morphology in Kupffer's vesicle and the establishment of expression in the lateral plate mesoderm. Collectively, our data indicate that is critical for robust Nodal signaling at multiple stages in zebrafish embryonic development.</p>

DOI10.7554/eLife.28635
Alternate JournalElife
PubMed ID29140250
PubMed Central IDPMC5745080
Grant ListR01 HD048584 / HD / NICHD NIH HHS / United States
T32 GM007388 / GM / NIGMS NIH HHS / United States