The gain-of-function allele bypasses the essential requirement for BamD in β-barrel outer membrane protein assembly.

TitleThe gain-of-function allele bypasses the essential requirement for BamD in β-barrel outer membrane protein assembly.
Publication TypeJournal Article
Year of Publication2020
AuthorsHart, EM, Gupta, M, Wühr, M, Silhavy, TJ
JournalProc Natl Acad Sci U S A
Volume117
Issue31
Pagination18737-18743
Date Published2020 08 04
ISSN1091-6490
Abstract

<p>The outer membrane (OM) of gram-negative bacteria confers innate resistance to toxins and antibiotics. Integral β-barrel outer membrane proteins (OMPs) function to establish and maintain the selective permeability of the OM. OMPs are assembled into the OM by the β-barrel assembly machine (BAM), which is composed of one OMP-BamA-and four lipoproteins-BamB, C, D, and E. BamB, C, and E can be removed individually with only minor effects on barrier function; however, depletion of either BamA or BamD causes a global defect in OMP assembly and results in cell death. We have identified a gain-of-function mutation, , that bypasses the requirement for BamD. Although ::kan cells exhibit growth and OM barrier defects, they assemble OMPs with surprising robustness. Our results demonstrate that BamD does not play a catalytic role in OMP assembly, but rather functions to regulate the activity of BamA.</p>

DOI10.1073/pnas.2007696117
Alternate JournalProc. Natl. Acad. Sci. U.S.A.
PubMed ID32675245
PubMed Central IDPMC7414184
Grant ListR35 GM128813 / GM / NIGMS NIH HHS / United States
R35 GM118024 / GM / NIGMS NIH HHS / United States
R01 GM034821 / GM / NIGMS NIH HHS / United States