Functional architecture of MFS D-glucose transporters.

TitleFunctional architecture of MFS D-glucose transporters.
Publication TypeJournal Article
Year of Publication2014
AuthorsM Madej, G, Sun, L, Yan, N, H Kaback, R
JournalProc Natl Acad Sci U S A
Volume111
Issue7
PaginationE719-27
Date Published2014 Feb 18
ISSN1091-6490
KeywordsBase Sequence, Binding Sites, Biological Transport, Calorimetry, DNA, Complementary, Escherichia coli O157, Escherichia coli Proteins, Glucose Transport Proteins, Facilitative, Humans, Liposomes, Models, Molecular, Molecular Sequence Data, Protein Conformation, Sequence Alignment, Sequence Analysis, DNA, Sequence Homology, Substrate Specificity, Symporters
Abstract

The Major Facilitator Superfamily (MFS) is a diverse group of secondary transporters with over 10,000 members, found in all kingdoms of life, including Homo sapiens. One objective of determining crystallographic models of the bacterial representatives is identification and physical localization of residues important for catalysis in transporters with medical relevance. The recently solved crystallographic models of the D-xylose permease XylE from Escherichia coli and GlcP from Staphylococcus epidermidus, homologs of the human D-glucose transporters, the GLUTs (SLC2), provide information about the structure of these transporters. The goal of this work is to examine general concepts derived from the bacterial XylE, GlcP, and other MFS transporters for their relevance to the GLUTs by comparing conservation of functionally critical residues. An energy landscape for symport and uniport is presented. Furthermore, the substrate selectivity of XylE is compared with GLUT1 and GLUT5, as well as a XylE mutant that transports D-glucose.

DOI10.1073/pnas.1400336111
Alternate JournalProc. Natl. Acad. Sci. U.S.A.
PubMed ID24550316
PubMed Central IDPMC3932877
Grant ListR56 DK051131 / DK / NIDDK NIH HHS / United States
GM073210 / GM / NIGMS NIH HHS / United States
DK069463 / DK / NIDDK NIH HHS / United States
DK51131 / DK / NIDDK NIH HHS / United States
R01 DK051131 / DK / NIDDK NIH HHS / United States
P50 GM073210 / GM / NIGMS NIH HHS / United States
R56 DK069463 / DK / NIDDK NIH HHS / United States
R01 DK069463 / DK / NIDDK NIH HHS / United States