Fluoroquinolone Persistence in Requires DNA Repair despite Differing between Starving Populations.

TitleFluoroquinolone Persistence in Requires DNA Repair despite Differing between Starving Populations.
Publication TypeJournal Article
Year of Publication2022
AuthorsLemma, AS, Soto-Echevarria, N, Brynildsen, MP
JournalMicroorganisms
Volume10
Issue2
Date Published2022/01/26
ISSN2076-2607
Abstract

When faced with nutritional deprivation, bacteria undergo a range of metabolic, regulatory, and biosynthetic changes. Those adjustments, which can be specific or independent of the missing nutrient, often alter bacterial tolerance to antibiotics. Here, using fluoroquinolones, we quantified persister levels in cultures experiencing starvation from a lack of carbon (C), nitrogen (N), phosphorous (P), or magnesium (Mg). Interestingly, persister levels varied significantly based on the type of starvation as well as fluoroquinolone used with N-starved populations exhibiting the highest persistence to levofloxacin, and P-starved populations exhibiting the highest persistence to moxifloxacin. However, regardless of the type of starvation or fluoroquinolone used, DNA repair was required by persisters, with ∆ and ∆ uniformly exhibiting the lowest persistence of the mutants assayed. These results suggest that while the type of starvation and fluoroquinolone will modulate the level of persistence, the importance of homologous recombination is consistently observed, which provides further support for efforts to target homologous recombination for anti-persister purposes.

DOI10.3390/microorganisms10020286
Alternate JournalMicroorganisms
PubMed ID35208744
PubMed Central IDPMC8877308
Grant ListR01 AI130293 / AI / NIAID NIH HHS / United States
T32 GM007388 / GM / NIGMS NIH HHS / United States
T32GM007388 / GM / NIGMS NIH HHS / United States
R01AI130293 / / National Institute of Allergy and Infectious Diseases /