Flow-Driven Rapid Vesicle Fusion via Vortex Trapping.

TitleFlow-Driven Rapid Vesicle Fusion via Vortex Trapping.
Publication TypeJournal Article
Year of Publication2015
AuthorsShin, S, Ault, JT, Stone, HA
JournalLangmuir
Volume31
Issue26
Pagination7178-82
Date Published2015 Jul 07
ISSN1520-5827
KeywordsHydrodynamics, Mechanical Phenomena, Stress, Mechanical, Unilamellar Liposomes
Abstract

Fusion between suspended lipid vesicles is difficult to achieve without membrane proteins or ions because the vesicles have extremely low equilibrium membrane tension and high poration energy. Nonetheless, vesicle fusion in the absence of mediators can also be achieved by mechanical forcing that is strong enough to induce membrane poration. Here, we employ a strong fluid shear stress to achieve vesicle fusion. By utilizing a unique vortex formation phenomenon in branched channels as a platform for capturing, stressing, and fusing the lipid vesicles, we directly visualize using high-speed imaging the vesicle fusion events, induced solely by shear, on the time scale of submilliseconds. We show that a large vesicle with a size of up to ∼10 μm can be achieved by the fusion of nanoscale vesicles. This technique has the potential to be utilized as a fast and simple way to produce giant unilamellar vesicles and to serve as a platform for visualizing vesicle interactions and fusions in the presence of shear.

DOI10.1021/acs.langmuir.5b01752
Alternate JournalLangmuir
PubMed ID26098933