A Flexible Platform for Monitoring Cerebellum-Dependent Sensory Associative Learning. Author Gerard Broussard, Mikhail Kislin, Caroline Jung, Samuel Wang Publication Year 2022 Type Journal Article Abstract Climbing fiber inputs to Purkinje cells provide instructive signals critical for cerebellum-dependent associative learning. Studying these signals in head-fixed mice facilitates the use of imaging, electrophysiological, and optogenetic methods. Here, a low-cost behavioral platform (~$1000) was developed that allows tracking of associative learning in head-fixed mice that locomote freely on a running wheel. The platform incorporates two common associative learning paradigms: eyeblink conditioning and delayed tactile startle conditioning. Behavior is tracked using a camera and the wheel movement by a detector. We describe the components and setup and provide a detailed protocol for training and data analysis. This platform allows the incorporation of optogenetic stimulation and fluorescence imaging. The design allows a single host computer to control multiple platforms for training multiple animals simultaneously. Keywords Animals, Cerebellum, Mice, Purkinje Cells, Blinking, Optogenetics, Conditioning, Classical Journal J Vis Exp Issue 179 Date Published 2022 Jan 19 ISSN Number 1940-087X DOI 10.3791/63205 Alternate Journal J Vis Exp PMCID PMC9118201 PMID 35129170 PubMedPubMed CentralGoogle ScholarBibTeXEndNote X3 XML