A Flexible Platform for Monitoring Cerebellum-Dependent Sensory Associative Learning.

TitleA Flexible Platform for Monitoring Cerebellum-Dependent Sensory Associative Learning.
Publication TypeJournal Article
Year of Publication2022
AuthorsBroussard, GJoey, Kislin, M, Jung, C, Wang, SS-H
JournalJ Vis Exp
Issue179
Date Published2022 01 19
ISSN1940-087X
KeywordsAnimals, Blinking, Cerebellum, Conditioning, Classical, Mice, Optogenetics, Purkinje Cells
Abstract

<p>Climbing fiber inputs to Purkinje cells provide instructive signals critical for cerebellum-dependent associative learning. Studying these signals in head-fixed mice facilitates the use of imaging, electrophysiological, and optogenetic methods. Here, a low-cost behavioral platform (~$1000) was developed that allows tracking of associative learning in head-fixed mice that locomote freely on a running wheel. The platform incorporates two common associative learning paradigms: eyeblink conditioning and delayed tactile startle conditioning. Behavior is tracked using a camera and the wheel movement by a detector. We describe the components and setup and provide a detailed protocol for training and data analysis. This platform allows the incorporation of optogenetic stimulation and fluorescence imaging. The design allows a single host computer to control multiple platforms for training multiple animals simultaneously.</p>

DOI10.3791/63205
Alternate JournalJ Vis Exp
PubMed ID35129170
PubMed Central IDPMC9118201
Grant ListF32 MH120887 / MH / NIMH NIH HHS / United States
R01 MH115750 / MH / NIMH NIH HHS / United States
R01 NS045193 / NS / NINDS NIH HHS / United States