Evidence for a signaling system in Helicobacter pylori: detection of a luxS-encoded autoinducer.

TitleEvidence for a signaling system in Helicobacter pylori: detection of a luxS-encoded autoinducer.
Publication TypeJournal Article
Year of Publication2000
AuthorsJoyce, EA, Bassler, BL, Wright, A
JournalJ Bacteriol
Volume182
Issue13
Pagination3638-43
Date Published2000 Jul
ISSN0021-9193
KeywordsAmino Acid Sequence, Bacterial Proteins, Carbon-Sulfur Lyases, Electrophoresis, Gel, Two-Dimensional, Gene Expression, Helicobacter pylori, Humans, Molecular Sequence Data, Mutagenesis, Sequence Homology, Amino Acid, Tumor Cells, Cultured, Virulence
Abstract

<p>Helicobacter pylori possesses a homolog of the luxS gene, initially identified by its role in autoinducer production for the quorum-sensing system 2 in Vibrio harveyi. The genomes of several other species of bacteria, notably Escherichia coli, Salmonella enterica serovar Typhimurium, and Vibrio cholerae, also include luxS homologs. All of these bacteria have been shown to produce active autoinducers capable of stimulating the expression of the luciferase operon in V. harveyi. In this report, we demonstrate that H. pylori also synthesizes a functional autoinducer (AI-2) that can specifically activate signaling system 2 in V. harveyi. Maximal activity is produced during early log phase, and the activity is diminished when cells enter stationary phase. We show that AI-2 is not involved in modulating any of the known or putative virulence factors in H. pylori and that a luxS null mutant has a two-dimensional protein profile identical to that of its isogenic parent strain. We discuss the implications of having an AI-2-like quorum-sensing system in H. pylori and suggest possible roles that it may play in H. pylori infection.</p>

Alternate JournalJ. Bacteriol.
PubMed ID10850976
PubMed Central IDPMC94532
Grant ListP30 DK034928 / DK / NIDDK NIH HHS / United States
P30DK34928 / DK / NIDDK NIH HHS / United States