Establishment of a Developmental Compartment Requires Interactions between Three Synergistic Cis-regulatory Modules.

TitleEstablishment of a Developmental Compartment Requires Interactions between Three Synergistic Cis-regulatory Modules.
Publication TypeJournal Article
Year of Publication2015
AuthorsBieli, D, Kanca, O, Requena, D, Hamaratoglu, F, Gohl, D, Schedl, P, Affolter, M, Slattery, M, Müller, M, Estella, C
JournalPLoS Genet
Volume11
Issue10
Paginatione1005376
Date Published2015 Oct
ISSN1553-7404
KeywordsAnimals, Body Patterning, Cell Lineage, Drosophila melanogaster, Drosophila Proteins, Enhancer Elements, Genetic, Gene Expression Regulation, Developmental, Homeodomain Proteins, Imaginal Discs, LIM-Homeodomain Proteins, Regulatory Elements, Transcriptional, Regulatory Sequences, Nucleic Acid, Transcription Factors, Wings, Animal
Abstract

<p>The subdivision of cell populations in compartments is a key event during animal development. In Drosophila, the gene apterous (ap) divides the wing imaginal disc in dorsal vs ventral cell lineages and is required for wing formation. ap function as a dorsal selector gene has been extensively studied. However, the regulation of its expression during wing development is poorly understood. In this study, we analyzed ap transcriptional regulation at the endogenous locus and identified three cis-regulatory modules (CRMs) essential for wing development. Only when the three CRMs are combined, robust ap expression is obtained. In addition, we genetically and molecularly analyzed the trans-factors that regulate these CRMs. Our results propose a three-step mechanism for the cell lineage compartment expression of ap that includes initial activation, positive autoregulation and Trithorax-mediated maintenance through separable CRMs. </p>

DOI10.1371/journal.pgen.1005376
Alternate JournalPLoS Genet.
PubMed ID26468882
PubMed Central IDPMC4607503
Grant ListR01 GM043432 / GM / NIGMS NIH HHS / United States