Title | ER-associated retrograde SNAREs and the Dsl1 complex mediate an alternative, Sey1p-independent homotypic ER fusion pathway. |
Publication Type | Journal Article |
Year of Publication | 2014 |
Authors | Rogers, JV, McMahon, C, Baryshnikova, A, Hughson, FM, Rose, MD |
Journal | Mol Biol Cell |
Volume | 25 |
Issue | 21 |
Pagination | 3401-12 |
Date Published | 2014 Nov 01 |
ISSN | 1939-4586 |
Keywords | Coat Protein Complex I, Endoplasmic Reticulum, Multiprotein Complexes, Mutation, Saccharomyces cerevisiae Proteins, SNARE Proteins, Vesicular Transport Proteins |
Abstract | <p>The peripheral endoplasmic reticulum (ER) network is dynamically maintained by homotypic (ER-ER) fusion. In Saccharomyces cerevisiae, the dynamin-like GTPase Sey1p can mediate ER-ER fusion, but sey1Δ cells have no growth defect and only slightly perturbed ER structure. Recent work suggested that ER-localized soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) mediate a Sey1p-independent ER-ER fusion pathway. However, an alternative explanation--that the observed phenotypes arose from perturbed vesicle trafficking--could not be ruled out. In this study, we used candidate and synthetic genetic array (SGA) approaches to more fully characterize SNARE-mediated ER-ER fusion. We found that Dsl1 complex mutations in sey1Δ cells cause strong synthetic growth and ER structure defects and delayed ER-ER fusion in vivo, additionally implicating the Dsl1 complex in SNARE-mediated ER-ER fusion. In contrast, cytosolic coat protein I (COPI) vesicle coat mutations in sey1Δ cells caused no synthetic defects, excluding perturbed retrograde trafficking as a cause for the previously observed synthetic defects. Finally, deleting the reticulons that help maintain ER architecture in cells disrupted for both ER-ER fusion pathways caused almost complete inviability. We conclude that the ER SNAREs and the Dsl1 complex directly mediate Sey1p-independent ER-ER fusion and that, in the absence of both pathways, cell viability depends upon membrane curvature-promoting reticulons.</p> |
DOI | 10.1091/mbc.E14-07-1220 |
Alternate Journal | Mol Biol Cell |
PubMed ID | 25187651 |
PubMed Central ID | PMC4214786 |
Grant List | R01 GM037739 / GM / NIGMS NIH HHS / United States GM007388 / GM / NIGMS NIH HHS / United States GM037739 / GM / NIGMS NIH HHS / United States T32 GM007388 / GM / NIGMS NIH HHS / United States R01 GM071574 / GM / NIGMS NIH HHS / United States GM071574 / GM / NIGMS NIH HHS / United States |