Title | Epithelial geometry regulates spindle orientation and progenitor fate during formation of the mammalian epidermis. |
Publication Type | Journal Article |
Year of Publication | 2019 |
Authors | Box, K, Joyce, BW, Devenport, D |
Journal | Elife |
Volume | 8 |
Date Published | 2019 06 12 |
ISSN | 2050-084X |
Keywords | Animals, Cell Division, Cell Lineage, Cell Polarity, Cell Shape, Embryo, Mammalian, Epidermal Cells, Epidermis, Epithelium, Mammals, Mice, Inbred C57BL, Mutation, Nerve Tissue Proteins, Neural Tube, Spindle Apparatus, Stem Cells |
Abstract | <p>The control of cell fate through oriented cell division is imperative for proper organ development. Basal epidermal progenitor cells divide parallel or perpendicular to the basement membrane to self-renew or produce differentiated stratified layers, but the mechanisms regulating the choice between division orientations are unknown. Using time-lapse imaging to follow divisions and fates of basal progenitors, we find that mouse embryos defective for the planar cell polarity (PCP) gene, , exhibit increased perpendicular divisions and hyperthickened epidermis. Surprisingly, this is not due to defective Vangl2 function in the epidermis, but to changes in cell geometry and packing that arise from the open neural tube characteristic of PCP mutants. Through regional variations in epidermal deformation and physical manipulations, we show that local tissue architecture, rather than cortical PCP cues, regulates the decision between symmetric and stratifying divisions, allowing flexibility for basal cells to adapt to the needs of the developing tissue.</p> |
DOI | 10.7554/eLife.47102 |
Alternate Journal | Elife |
PubMed ID | 31187731 |
PubMed Central ID | PMC6592681 |
Grant List | T32GM007388 / NH / NIH HHS / United States R01AR068320 / NH / NIH HHS / United States AR8472471 / NH / NIH HHS / United States R01 AR068320 / AR / NIAMS NIH HHS / United States T32 GM007388 / GM / NIGMS NIH HHS / United States |