Epithelial and mesenchymal prostate cancer cell population dynamics on a complex drug landscape.

TitleEpithelial and mesenchymal prostate cancer cell population dynamics on a complex drug landscape.
Publication TypeJournal Article
Year of Publication2017
AuthorsLin, K-C, Torga, G, Wu, A, Rabinowitz, JD, Murray, WJ, Sturm, JC, Pienta, KJ, Austin, R
JournalConverg Sci Phys Oncol
Volume3
Issue4
Date Published2017 Dec
ISSN2057-1739
Abstract

We have improved our microfluidic cell culture device that generates an landscape of stress heterogeneity. We now can do continuous observations of different cancer cell lines and carry out downstream analysis of cell phenotype as a function of position on the stress landscape. We use this technology to probe adaption and evolution dynamics in prostate cancer cell metapopulations under a stress landscape of a chemotherapeutic drug (docetaxel). The utility of this approach is highlighted by analysis of heterogenous prostate cancer cell motility changes as a function of position in the stress landscape. Because the technology presented here is easily adapted to a standard epifluorescence microscope it has the potential for broad application in preclinical drug development and assays of likely drug efficacy.

Alternate JournalConverg Sci Phys Oncol
PubMed ID29527324
PubMed Central IDPMC5840799
Grant ListU01 CA143055 / CA / NCI NIH HHS / United States
R01 CA163214 / CA / NCI NIH HHS / United States
U54 CA143803 / CA / NCI NIH HHS / United States
P50 CA069568 / CA / NCI NIH HHS / United States
P01 CA093900 / CA / NCI NIH HHS / United States