Enhancer Control of Transcriptional Bursting.

TitleEnhancer Control of Transcriptional Bursting.
Publication TypeJournal Article
Year of Publication2016
AuthorsFukaya, T, Lim, B, Levine, M
JournalCell
Volume166
Issue2
Pagination358-68
Date Published2016 Jul 14
ISSN1097-4172
Abstract

<p>Transcription is episodic, consisting of a series of discontinuous bursts. Using live-imaging methods and quantitative analysis, we examine transcriptional bursting in living Drosophila embryos. Different developmental enhancers positioned downstream of synthetic reporter genes produce transcriptional bursts with similar amplitudes and duration but generate very different bursting frequencies, with strong enhancers producing more bursts than weak enhancers. Insertion of an insulator reduces the number of bursts and the corresponding level of gene expression, suggesting that enhancer regulation of bursting frequency is a key parameter of gene control in development. We also show that linked reporter genes exhibit coordinated bursting profiles when regulated by a shared enhancer, challenging conventional models of enhancer-promoter looping.</p>

DOI10.1016/j.cell.2016.05.025
Alternate JournalCell
PubMed ID27293191
PubMed Central IDPMC4970759
Grant ListR01 GM034431 / GM / NIGMS NIH HHS / United States
R01 GM046638 / GM / NIGMS NIH HHS / United States
R37 GM046638 / GM / NIGMS NIH HHS / United States
U01 EB021239 / EB / NIBIB NIH HHS / United States