Employing NaChBac for cryo-EM analysis of toxin action on voltage-gated Na channels in nanodisc.

TitleEmploying NaChBac for cryo-EM analysis of toxin action on voltage-gated Na channels in nanodisc.
Publication TypeJournal Article
Year of Publication2020
AuthorsGao, S, Valinsky, WC, On, NCam, Houlihan, PR, Qu, Q, Liu, L, Pan, X, Clapham, DE, Yan, N
JournalProc Natl Acad Sci U S A
Volume117
Issue25
Pagination14187-14193
Date Published2020 06 23
ISSN1091-6490
KeywordsAnimals, Bacterial Proteins, Cryoelectron Microscopy, Humans, Lipid Bilayers, Models, Molecular, Nanostructures, Protein Conformation, Sodium Channels, Spider Venoms, Voltage-Gated Sodium Channels
Abstract

<p>NaChBac, the first bacterial voltage-gated Na (Na) channel to be characterized, has been the prokaryotic prototype for studying the structure-function relationship of Na channels. Discovered nearly two decades ago, the structure of NaChBac has not been determined. Here we present the single particle electron cryomicroscopy (cryo-EM) analysis of NaChBac in both detergent micelles and nanodiscs. Under both conditions, the conformation of NaChBac is nearly identical to that of the potentially inactivated NaAb. Determining the structure of NaChBac in nanodiscs enabled us to examine gating modifier toxins (GMTs) of Na channels in lipid bilayers. To study GMTs in mammalian Na channels, we generated a chimera in which the extracellular fragment of the S3 and S4 segments in the second voltage-sensing domain from Na1.7 replaced the corresponding sequence in NaChBac. Cryo-EM structures of the nanodisc-embedded chimera alone and in complex with HuwenToxin IV (HWTX-IV) were determined to 3.5 and 3.2 Å resolutions, respectively. Compared to the structure of HWTX-IV-bound human Na1.7, which was obtained at an overall resolution of 3.2 Å, the local resolution of the toxin has been improved from ∼6 to ∼4 Å. This resolution enabled visualization of toxin docking. NaChBac can thus serve as a convenient surrogate for structural studies of the interactions between GMTs and Na channels in a membrane environment.</p>

DOI10.1073/pnas.1922903117
Alternate JournalProc. Natl. Acad. Sci. U.S.A.
PubMed ID32513729
PubMed Central IDPMC7322032
Grant ListR01 GM130762 / GM / NIGMS NIH HHS / United States