An Easy-to-Fabricate Cell Stretcher Reveals Density-Dependent Mechanical Regulation of Collective Cell Movements in Epithelia.

TitleAn Easy-to-Fabricate Cell Stretcher Reveals Density-Dependent Mechanical Regulation of Collective Cell Movements in Epithelia.
Publication TypeJournal Article
Year of Publication2021
AuthorsHart, KC, Sim, JYong, Hopcroft, MA, Cohen, DJ, Tan, J, W Nelson, J, Pruitt, BL
JournalCell Mol Bioeng
Volume14
Issue6
Pagination569-581
Date Published2021 Dec
ISSN1865-5025
Abstract

<p><b>INTRODUCTION: </b>Mechanical forces regulate many facets of cell and tissue biology. Studying the effects of forces on cells requires real-time observations of single- and multi-cell dynamics in tissue models during controlled external mechanical input. Many of the existing devices used to conduct these studies are costly and complicated to fabricate, which reduces the availability of these devices to many laboratories.</p><p><b>METHODS: </b>We show how to fabricate a simple, low-cost, uniaxial stretching device, with readily available materials and instruments that is compatible with high-resolution time-lapse microscopy of adherent cell monolayers. In addition, we show how to construct a pressure controller that induces a repeatable degree of stretch in monolayers, as well as a custom MATLAB code to quantify individual cell strains.</p><p><b>RESULTS: </b>As an application note using this device, we show that uniaxial stretch slows down cellular movements in a mammalian epithelial monolayer in a cell density-dependent manner. We demonstrate that the effect on cell movement involves the relocalization of myosin downstream of Rho-associated protein kinase (ROCK).</p><p><b>CONCLUSIONS: </b>This mechanical device provides a platform for broader involvement of engineers and biologists in this important area of cell and tissue biology. We used this device to demonstrate the mechanical regulation of collective cell movements in epithelia.</p><p><b>SUPPLEMENTARY INFORMATION: </b>The online version contains supplementary material available at 10.1007/s12195-021-00689-6.</p>

DOI10.1007/s12195-021-00689-6
Alternate JournalCell Mol Bioeng
PubMed ID34900011
PubMed Central IDPMC8630312