Dynamics of gradient formation by intracellular shuttling.

TitleDynamics of gradient formation by intracellular shuttling.
Publication TypeJournal Article
Year of Publication2015
AuthorsBerezhkovskii, AM, Shvartsman, SY
JournalJ Chem Phys
Volume143
Issue7
Pagination074116
Date Published2015 Aug 21
ISSN1089-7690
KeywordsBiological Transport, Computer Simulation, Enzymes, Intracellular Space, Kinetics, Membrane Proteins, Models, Biological
Abstract

<p>A number of important cellular functions rely on the formation of intracellular protein concentration gradients. Experimental studies discovered a number of mechanisms for the formation of such gradients. One of the mechanisms relies on the intracellular shuttling of a protein that interconverts between the two states with different diffusivities, under the action of two enzymes, one of which is localized to the plasma membrane, whereas the second is uniformly distributed in the cytoplasm. Recent work reported an analytical solution for the steady state gradient in this mechanism, obtained in the framework of a one-dimensional reaction-diffusion model. Here, we study the dynamics in this model and derive analytical expressions for the Laplace transforms of the time-dependent concentration profiles in terms of elementary transcendental functions. Inverting these transforms numerically, one can obtain time-dependent concentration profiles of the two forms of the protein. </p>

DOI10.1063/1.4928858
Alternate JournalJ Chem Phys
PubMed ID26298124
PubMed Central IDPMC5562396
Grant List / / Intramural NIH HHS / United States