Dynamics of endoderm specification.

TitleDynamics of endoderm specification.
Publication TypeJournal Article
Year of Publication2022
AuthorsKeenan, SE, Avdeeva, M, Yang, L, Alber, DS, Wieschaus, EF, Shvartsman, SY
JournalProc Natl Acad Sci U S A
Volume119
Issue15
Paginatione2112892119
Date Published2022 Apr 12
ISSN1091-6490
KeywordsAnimals, Body Patterning, Drosophila melanogaster, Drosophila Proteins, Endoderm, Gene Expression Regulation, Developmental, Gene Regulatory Networks
Abstract

<p>During early Drosophila embryogenesis, a network of gene regulatory interactions orchestrates terminal patterning, playing a critical role in the subsequent formation of the gut. We utilized CRISPR gene editing at endogenous loci to create live reporters of transcription and light-sheet microscopy to monitor the individual components of the posterior gut patterning network across 90 min prior to gastrulation. We developed a computational approach for fusing imaging datasets of the individual components into a common multivariable trajectory. Data fusion revealed low intrinsic dimensionality of posterior patterning and cell fate specification in wild-type embryos. The simple structure that we uncovered allowed us to construct a model of interactions within the posterior patterning regulatory network and make testable predictions about its dynamics at the protein level. The presented data fusion strategy is a step toward establishing a unified framework that would explore how stochastic spatiotemporal signals give rise to highly reproducible morphogenetic outcomes.</p>

DOI10.1073/pnas.2112892119
Alternate JournalProc Natl Acad Sci U S A
PubMed ID35412853
PubMed Central IDPMC9169638
Grant ListR01 HD085870 / HD / NICHD NIH HHS / United States
F32 GM119297 / GM / NIGMS NIH HHS / United States
R01 GM134204 / GM / NIGMS NIH HHS / United States
DP2 EB024247 / EB / NIBIB NIH HHS / United States