Dynamic regulation of eve stripe 2 expression reveals transcriptional bursts in living Drosophila embryos.

TitleDynamic regulation of eve stripe 2 expression reveals transcriptional bursts in living Drosophila embryos.
Publication TypeJournal Article
Year of Publication2014
AuthorsBothma, JP, Garcia, HG, Esposito, E, Schlissel, G, Gregor, T, Levine, M
JournalProc Natl Acad Sci U S A
Volume111
Issue29
Pagination10598-603
Date Published2014 Jul 22
ISSN1091-6490
KeywordsAnimals, Cell Nucleus, Drosophila melanogaster, Drosophila Proteins, Embryo, Nonmammalian, Female, Gene Expression Regulation, Developmental, Homeodomain Proteins, RNA, Messenger, Transcription Factors, Transcription, Genetic
Abstract

<p>We present the use of recently developed live imaging methods to examine the dynamic regulation of even-skipped (eve) stripe 2 expression in the precellular Drosophila embryo. Nascent transcripts were visualized via MS2 RNA stem loops. The eve stripe 2 transgene exhibits a highly dynamic pattern of de novo transcription, beginning with a broad domain of expression during nuclear cycle 12 (nc12), and progressive refinement during nc13 and nc14. The mature stripe 2 pattern is surprisingly transient, constituting just ∼15 min of the ∼90-min period of expression. Nonetheless, this dynamic transcription profile faithfully predicts the limits of the mature stripe visualized by conventional in situ detection methods. Analysis of individual transcription foci reveals intermittent bursts of de novo transcription, with duration cycles of 4-10 min. We discuss a multistate model of transcription regulation and speculate on its role in the dynamic repression of the eve stripe 2 expression pattern during development. </p>

DOI10.1073/pnas.1410022111
Alternate JournalProc. Natl. Acad. Sci. U.S.A.
PubMed ID24994903
PubMed Central IDPMC4115566
Grant ListR01 GM34431 / GM / NIGMS NIH HHS / United States
R01 GM034431 / GM / NIGMS NIH HHS / United States
P50 GM071508 / GM / NIGMS NIH HHS / United States
R01 GM097275 / GM / NIGMS NIH HHS / United States
T32 GM007127 / GM / NIGMS NIH HHS / United States