Title | The Drosophila hnRNP F/H Homolog Glorund Uses Two Distinct RNA-Binding Modes to Diversify Target Recognition. |
Publication Type | Journal Article |
Year of Publication | 2017 |
Authors | Tamayo, JV, Teramoto, T, Chatterjee, S, Hall, TMTanaka, Gavis, ER |
Journal | Cell Rep |
Volume | 19 |
Issue | 1 |
Pagination | 150-161 |
Date Published | 2017 Apr 04 |
ISSN | 2211-1247 |
Keywords | Alternative Splicing, Amino Acid Sequence, Animals, Drosophila melanogaster, Drosophila Proteins, Female, Heterogeneous-Nuclear Ribonucleoprotein Group F-H, Mutation, Nucleotide Motifs, Oocytes, Ovary, Protein Biosynthesis, RNA, RNA-Binding Proteins, Transforming Growth Factor alpha |
Abstract | <p>The Drosophila hnRNP F/H homolog, Glorund (Glo), regulates nanos mRNA translation by interacting with a structured UA-rich motif in the nanos 3' untranslated region. Glo regulates additional RNAs, however, and mammalian homologs bind G-tract sequences to regulate alternative splicing, suggesting that Glo also recognizes G-tract RNA. To gain insight into how Glo recognizes both structured UA-rich and G-tract RNAs, we used mutational analysis guided by crystal structures of Glo's RNA-binding domains and identified two discrete RNA-binding surfaces that allow Glo to recognize both RNA motifs. By engineering Glo variants that favor a single RNA-binding mode, we show that a subset of Glo's functions in vivo is mediated solely by the G-tract binding mode, whereas regulation of nanos requires both recognition modes. Our findings suggest a molecular mechanism for the evolution of dual RNA motif recognition in Glo that may be applied to understanding the functional diversity of other RNA-binding proteins.</p> |
DOI | 10.1016/j.celrep.2017.03.022 |
Alternate Journal | Cell Rep |
PubMed ID | 28380354 |
PubMed Central ID | PMC5392723 |
Grant List | R01 GM061107 / GM / NIGMS NIH HHS / United States ZIA ES050165-19 / / Intramural NIH HHS / United States |