Drosophila Dosage Compensation Loci Associate with a Boundary-Forming Insulator Complex.

TitleDrosophila Dosage Compensation Loci Associate with a Boundary-Forming Insulator Complex.
Publication TypeJournal Article
Year of Publication2017
AuthorsKaye, EG, Kurbidaeva, A, Wolle, D, Aoki, T, Schedl, P, Larschan, E
JournalMol Cell Biol
Volume37
Issue21
Date Published2017 Nov 01
ISSN1098-5549
KeywordsAnimals, Dosage Compensation, Genetic, Drosophila melanogaster, Drosophila Proteins, Genes, X-Linked, Genetic Loci, Insulator Elements, Male, Mutation, X Chromosome
Abstract

<p>Chromatin entry sites (CES) are 100- to 1,500-bp elements that recruit male-specific lethal (MSL) complexes to the X chromosome to upregulate expression of X-linked genes in male flies. CES contain one or more ∼20-bp GA-rich sequences called MSL recognition elements (MREs) that are critical for dosage compensation. Recent studies indicate that CES also correspond to boundaries of X-chromosomal topologically associated domains (TADs). Here, we show that an ∼1,000-kDa complex called the late boundary complex (LBC), which is required for the functioning of the Bithorax complex boundary , interacts specifically with a special class of CES that contain multiple MREs. Mutations in the MRE sequences of three of these CES that disrupt function abrogate interactions with the LBC. Moreover, reducing the levels of two LBC components compromises MSL recruitment. Finally, we show that several of the CES that are physically linked to each other are LBC interactors.</p>

DOI10.1128/MCB.00253-17
Alternate JournalMol. Cell. Biol.
PubMed ID28784719
PubMed Central IDPMC5640815
Grant ListR01 GM043432 / GM / NIGMS NIH HHS / United States
R01 GM098461 / GM / NIGMS NIH HHS / United States
R56 GM043432 / GM / NIGMS NIH HHS / United States
T32 AG041688 / AG / NIA NIH HHS / United States