Divergent effects of intrinsically active MEK variants on developmental Ras signaling.

TitleDivergent effects of intrinsically active MEK variants on developmental Ras signaling.
Publication TypeJournal Article
Year of Publication2017
AuthorsGoyal, Y, Jindal, GA, Pelliccia, JL, Yamaya, K, Yeung, E, Futran, AS, Burdine, RD, Schüpbach, T, Shvartsman, SY
JournalNat Genet
Date Published2017 Mar
KeywordsAnimals, Drosophila melanogaster, Germ-Line Mutation, Heart Diseases, Humans, Mitogen-Activated Protein Kinases, Neoplasms, Neurocognitive Disorders, Phenotype, ras Proteins, Signal Transduction, Zebrafish

<p>Germline mutations in Ras pathway components are associated with a large class of human developmental abnormalities, known as RASopathies, that are characterized by a range of structural and functional phenotypes, including cardiac defects and neurocognitive delays. Although it is generally believed that RASopathies are caused by altered levels of pathway activation, the signaling changes in developing tissues remain largely unknown. We used assays with spatiotemporal resolution in Drosophila melanogaster (fruit fly) and Danio rerio (zebrafish) to quantify signaling changes caused by mutations in MAP2K1 (encoding MEK), a core component of the Ras pathway that is mutated in both RASopathies and cancers in humans. Surprisingly, we discovered that intrinsically active MEK variants can both increase and reduce the levels of pathway activation in vivo. The sign of the effect depends on cellular context, implying that some of the emerging phenotypes in RASopathies may be caused by increased, as well as attenuated, levels of Ras signaling.</p>

Alternate JournalNat Genet
PubMed ID28166211
PubMed Central IDPMC5621734
Grant ListR01 GM077620 / GM / NIGMS NIH HHS / United States
R01 GM086537 / GM / NIGMS NIH HHS / United States
R01 HD048584 / HD / NICHD NIH HHS / United States
T32 GM007388 / GM / NIGMS NIH HHS / United States