Distinct cytoskeletal proteins define zones of enhanced cell wall synthesis in .

TitleDistinct cytoskeletal proteins define zones of enhanced cell wall synthesis in .
Publication TypeJournal Article
Year of Publication2020
AuthorsTaylor, JA, Bratton, BP, Sichel, SR, Blair, KM, Jacobs, HM, DeMeester, KE, Kuru, E, Gray, J, Biboy, J, VanNieuwenhze, MS, Vollmer, W, Grimes, CL, Shaevitz, JW, Salama, NR
JournalElife
Volume9
Date Published2020 Jan 09
ISSN2050-084X
Abstract

<p>Helical cell shape is necessary for efficient stomach colonization by , but the molecular mechanisms for generating helical shape remain unclear. The helical centerline pitch and radius of wild-type cells dictate surface curvatures of considerably higher positive and negative Gaussian curvatures than those present in straight- or curved-rod . Quantitative 3D microscopy analysis of short pulses with either -acetylmuramic acid or D-alanine metabolic probes showed that cell wall growth is enhanced at both sidewall curvature extremes. Immunofluorescence revealed MreB is most abundant at negative Gaussian curvature, while the bactofilin CcmA is most abundant at positive Gaussian curvature. Strains expressing CcmA variants with altered polymerization properties lose helical shape and associated positive Gaussian curvatures. We thus propose a model where CcmA and MreB promote PG synthesis at positive and negative Gaussian curvatures, respectively, and that this patterning is one mechanism necessary for maintaining helical shape.</p>

DOI10.7554/eLife.52482
Alternate JournalElife
PubMed ID31916938
PubMed Central IDPMC7012605
Grant ListT32 GM095421 / GM / NIGMS NIH HHS / United States
T32 GM008550 / GM / NIGMS NIH HHS / United States
T32 GM008550 / NH / NIH HHS / United States
DGE-0718124 / / National Science Foundation /
U01 CA221230 / NH / NIH HHS / United States
T32 GM95421 / NH / NIH HHS / United States
GM113172 / NH / NIH HHS / United States
R01 AI136946 / AI / NIAID NIH HHS / United States
PHY-1734030 / / National Science Foundation /
T32 CA009657 / CA / NCI NIH HHS / United States
R01 AI136946 / NH / NIH HHS / United States
P30 CA015704 / NH / NIH HHS / United States
R21 AI121828 / AI / NIAID NIH HHS / United States
Graduate Opportunity Program Research Assistantship Award / / Graduate Opportunities and Minority Achievement Program /
R21 AI121828 / NH / NIH HHS / United States
Graduate Opportunity Program Research Assistantship Award / / GO-MAP /
National Defense Science & Engineering Graduate Fellowship (NDSEG) / / Department of Defense /
P30 CA015704 / CA / NCI NIH HHS / United States
U01 CA221230 / CA / NCI NIH HHS / United States
Stanford Imaging Award Number 1S10OD01227601 / RR / NCRR NIH HHS / United States
T32 CA009657 / NH / NIH HHS / United States
DGE-1256082 / / National Science Foundation /
/ WT_ / Wellcome Trust / United Kingdom
R01 GM113172 / GM / NIGMS NIH HHS / United States
101824/Z/13/Z / / Wellcome /