Distinct -acting elements mediate targeting and clustering of polar granule mRNAs.

TitleDistinct -acting elements mediate targeting and clustering of polar granule mRNAs.
Publication TypeJournal Article
Year of Publication2018
AuthorsEagle, WVI, Yeboah-Kordieh, DK, Niepielko, MG, Gavis, ER
JournalDevelopment
Volume145
Issue22
Date Published2018 11 22
ISSN1477-9129
Keywords3' Untranslated Regions, Animals, Base Pairing, Cell Polarity, Conserved Sequence, Cytoplasmic Granules, Drosophila melanogaster, Drosophila Proteins, Intercellular Signaling Peptides and Proteins, Nuclear Proteins, Nucleotide Motifs, Positive Transcriptional Elongation Factor B, Regulatory Sequences, Nucleic Acid, RNA, Messenger
Abstract

<p>Specification and development of germ cells depend on molecular determinants within the germ plasm, a specialized cytoplasmic domain at the posterior of the embryo. Localization of numerous mRNAs to the germ plasm occurs by their incorporation, as single-transcript ribonucleoprotein (RNP) particles, into complex RNP granules called polar granules. Incorporation of mRNAs into polar granules is followed by recruitment of additional like transcripts to form discrete homotypic clusters. The -acting localization signals that target mRNAs to polar granules and promote homotypic clustering remain largely uncharacterized. Here, we show that the () and () 3' untranslated regions contain complex localization signals comprising multiple, independently weak and partially functionally redundant localization elements (LEs). We demonstrate that targeting of to polar granules and self-assembly into homotypic clusters are functionally separable processes mediated by distinct classes of LEs. We identify a sequence motif shared by other polar granule mRNAs that contributes to homotypic clustering. Our results suggest that mRNA localization signal complexity may be a feature required by the targeting and self-recruitment mechanism that drives germ plasm mRNA localization.</p>

DOI10.1242/dev.164657
Alternate JournalDevelopment
PubMed ID30333216
PubMed Central IDPMC6262787
Grant ListF32 GM119200 / GM / NIGMS NIH HHS / United States
R01 GM067758 / GM / NIGMS NIH HHS / United States
T32 GM007388 / GM / NIGMS NIH HHS / United States