Discovery of Ubonodin, an Antimicrobial Lasso Peptide Active against Members of the Burkholderia cepacia Complex. Author Wai Cheung-Lee, Madison Parry, Chuhan Zong, Alexis Cartagena, Seth Darst, Nancy Connell, Riccardo Russo, A James Link Publication Year 2020 Type Journal Article Abstract We report the heterologous expression, structure, and antimicrobial activity of a lasso peptide, ubonodin, encoded in the genome of Burkholderia ubonensis. The topology of ubonodin is unprecedented amongst lasso peptides, with 18 of its 28 amino acids found in the mechanically bonded loop segment. Ubonodin inhibits RNA polymerase in vitro and has potent antimicrobial activity against several pathogenic members of the Burkholderia genus, most notably B. cepacia and B. multivorans, causative agents of lung infections in cystic fibrosis patients. Keywords Anti-Bacterial Agents, Humans, DNA-Directed RNA Polymerases, Drug Discovery, Pore Forming Cytotoxic Proteins, Burkholderia cepacia complex Journal Chembiochem Volume 21 Issue 9 Pages 1335-1340 Date Published 2020 May 04 ISSN Number 1439-7633 DOI 10.1002/cbic.201900707 Alternate Journal Chembiochem PMCID PMC7205569 PMID 31765515 PubMedPubMed CentralGoogle ScholarBibTeXEndNote X3 XML