Title | Discovery of Ubonodin, an Antimicrobial Lasso Peptide Active against Members of the Burkholderia cepacia Complex. |
Publication Type | Journal Article |
Year of Publication | 2020 |
Authors | Cheung-Lee, WLing, Parry, ME, Zong, C, Cartagena, AJaramillo, Darst, SA, Connell, ND, Russo, R, A Link, J |
Journal | Chembiochem |
Volume | 21 |
Issue | 9 |
Pagination | 1335-1340 |
Date Published | 2020 May 04 |
ISSN | 1439-7633 |
Keywords | Anti-Bacterial Agents, Burkholderia cepacia complex, DNA-Directed RNA Polymerases, Drug Discovery, Humans, Pore Forming Cytotoxic Proteins |
Abstract | <p>We report the heterologous expression, structure, and antimicrobial activity of a lasso peptide, ubonodin, encoded in the genome of Burkholderia ubonensis. The topology of ubonodin is unprecedented amongst lasso peptides, with 18 of its 28 amino acids found in the mechanically bonded loop segment. Ubonodin inhibits RNA polymerase in vitro and has potent antimicrobial activity against several pathogenic members of the Burkholderia genus, most notably B. cepacia and B. multivorans, causative agents of lung infections in cystic fibrosis patients.</p> |
DOI | 10.1002/cbic.201900707 |
Alternate Journal | Chembiochem |
PubMed ID | 31765515 |
PubMed Central ID | PMC7205569 |
Grant List | R35 GM118130 / GM / NIGMS NIH HHS / United States R01 GM107036 / GM / NIGMS NIH HHS / United States |