Directional reversals enable Myxococcus xanthus cells to produce collective one-dimensional streams during fruiting-body formation.

TitleDirectional reversals enable Myxococcus xanthus cells to produce collective one-dimensional streams during fruiting-body formation.
Publication TypeJournal Article
Year of Publication2015
AuthorsThutupalli, S, Sun, M, Bunyak, F, Palaniappan, K, Shaevitz, JW
JournalJ R Soc Interface
Volume12
Issue109
Pagination20150049
Date Published2015 Aug 06
ISSN1742-5662
KeywordsModels, Biological, Myxococcus xanthus
Abstract

<p>The formation of a collectively moving group benefits individuals within a population in a variety of ways. The surface-dwelling bacterium Myxococcus xanthus forms dynamic collective groups both to feed on prey and to aggregate during times of starvation. The latter behaviour, termed fruiting-body formation, involves a complex, coordinated series of density changes that ultimately lead to three-dimensional aggregates comprising hundreds of thousands of cells and spores. How a loose, two-dimensional sheet of motile cells produces a fixed aggregate has remained a mystery as current models of aggregation are either inconsistent with experimental data or ultimately predict unstable structures that do not remain fixed in space. Here, we use high-resolution microscopy and computer vision software to spatio-temporally track the motion of thousands of individuals during the initial stages of fruiting-body formation. We find that cells undergo a phase transition from exploratory flocking, in which unstable cell groups move rapidly and coherently over long distances, to a reversal-mediated localization into one-dimensional growing streams that are inherently stable in space. These observations identify a new phase of active collective behaviour and answer a long-standing open question in Myxococcus development by describing how motile cell groups can remain statistically fixed in a spatial location. </p>

DOI10.1098/rsif.2015.0049
Alternate JournalJ R Soc Interface
PubMed ID26246416
PubMed Central IDPMC4535398
Grant ListP50GM071508 / GM / NIGMS NIH HHS / United States
R33-EB00573 / EB / NIBIB NIH HHS / United States