Direct evidence for cancer-cell-autonomous extracellular protein catabolism in pancreatic tumors.

TitleDirect evidence for cancer-cell-autonomous extracellular protein catabolism in pancreatic tumors.
Publication TypeJournal Article
Year of Publication2017
AuthorsDavidson, SM, Jonas, O, Keibler, MA, Hou, HWei, Luengo, A, Mayers, JR, Wyckoff, J, Del Rosario, AM, Whitman, M, Chin, CR, Condon, KJ, Lammers, A, Kellersberger, KA, Stall, BK, Stephanopoulos, G, Bar-Sagi, D, Han, J, Rabinowitz, JD, Cima, MJ, Langer, R, Heiden, MGVander
JournalNat Med
Date Published2017 Feb
KeywordsAlbumins, Amino Acids, Animals, Carcinoma, Pancreatic Ductal, Cell Line, Tumor, Chromatography, Gas, Disease Models, Animal, Extracellular Space, Mice, Microscopy, Fluorescence, Multiphoton, Nitrogen Isotopes, Pancreatic Neoplasms, Pinocytosis, Plasmapheresis, Proteins, Proteolysis, Serum Albumin, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

<p>Mammalian tissues rely on a variety of nutrients to support their physiological functions. It is known that altered metabolism is involved in the pathogenesis of cancer, but which nutrients support the inappropriate growth of intact malignant tumors is incompletely understood. Amino acids are essential nutrients for many cancer cells that can be obtained through the scavenging and catabolism of extracellular protein via macropinocytosis. In particular, macropinocytosis can be a nutrient source for pancreatic cancer cells, but it is not fully understood how the tumor environment influences metabolic phenotypes and whether macropinocytosis supports the maintenance of amino acid levels within pancreatic tumors. Here we utilize miniaturized plasma exchange to deliver labeled albumin to tissues in live mice, and we demonstrate that breakdown of albumin contributes to the supply of free amino acids in pancreatic tumors. We also deliver albumin directly into tumors using an implantable microdevice, which was adapted and modified from ref. 9. Following implantation, we directly observe protein catabolism and macropinocytosis in situ by pancreatic cancer cells, but not by adjacent, non-cancerous pancreatic tissue. In addition, we find that intratumoral inhibition of macropinocytosis decreases amino acid levels. Taken together, these data suggest that pancreatic cancer cells consume extracellular protein, including albumin, and that this consumption serves as an important source of amino acids for pancreatic cancer cells in vivo.</p>

Alternate JournalNat Med
PubMed ID28024083
PubMed Central IDPMC5407288
Grant ListDP1 CA174420 / CA / NCI NIH HHS / United States
T32 GM007287 / GM / NIGMS NIH HHS / United States
F30 CA183474 / CA / NCI NIH HHS / United States
R01 CA168653 / CA / NCI NIH HHS / United States
P30 CA014051 / CA / NCI NIH HHS / United States
T32 GM007753 / GM / NIGMS NIH HHS / United States
R01 CA160458 / CA / NCI NIH HHS / United States
R21 CA177391 / CA / NCI NIH HHS / United States
R01 DK075850 / DK / NIDDK NIH HHS / United States