Development of a pan-neuronal genetic driver in mosquitoes.

TitleDevelopment of a pan-neuronal genetic driver in mosquitoes.
Publication TypeJournal Article
Year of Publication2021
AuthorsZhao, Z, Tian, D, McBride, CS
JournalCell Rep Methods
Volume1
Issue3
Date Published2021 Jul 26
ISSN2667-2375
Abstract

<p>The recent development of neurogenetic tools in mosquitoes is beginning to shed light on the neural basis of behaviors that make this species a major vector of human disease. However, we still lack a pan-neuronal expression driver-a key tool that provides genetic access to all neurons. Here, we describe our efforts to fill this gap via CRISPR/Cas9-mediated knock-in of reporters to broadly expressed neural genes and report on the generation of two strains, a : strain that expresses synaptically localized GCaMP and a driver strain that can be used to drive and amplify expression of any effector via the Q binary system. Both manipulations broadly and uniformly label the nervous system with only modest effects on behavior. We expect these strains to facilitate neurobiological research in mosquitoes and document both successful and failed manipulations as a roadmap for similar tool development in other non-model species.</p>

DOI10.1016/j.crmeth.2021.100042
Alternate JournalCell Rep Methods
PubMed ID34590074
PubMed Central IDPMC8478256
Grant ListDP2 AI144246 / AI / NIAID NIH HHS / United States
R00 DC012069 / DC / NIDCD NIH HHS / United States