Development of light-responsive protein binding in the monobody non-immunoglobulin scaffold.

TitleDevelopment of light-responsive protein binding in the monobody non-immunoglobulin scaffold.
Publication TypeJournal Article
Year of Publication2020
AuthorsCarrasco-López, C, Zhao, EM, Gil, AA, Alam, N, Toettcher, JE, Avalos, JL
JournalNat Commun
Volume11
Issue1
Pagination4045
Date Published2020 08 13
ISSN2041-1723
KeywordsChromatography, Affinity, Escherichia coli, HEK293 Cells, Humans, Light, Optogenetics, Protein Binding, Proteins
Abstract

<p>Monobodies are synthetic non-immunoglobulin customizable protein binders invaluable to basic and applied research, and of considerable potential as future therapeutics and diagnostic tools. The ability to reversibly control their binding activity to their targets on demand would significantly expand their applications in biotechnology, medicine, and research. Here we present, as proof-of-principle, the development of a light-controlled monobody (OptoMB) that works in vitro and in cells and whose affinity for its SH2-domain target exhibits a 330-fold shift in binding affinity upon illumination. We demonstrate that our αSH2-OptoMB can be used to purify SH2-tagged proteins directly from crude E. coli extract, achieving 99.8% purity and over 40% yield in a single purification step. By virtue of their ability to be designed to bind any protein of interest, OptoMBs have the potential to find new powerful applications as light-switchable binders of untagged proteins with the temporal and spatial precision afforded by light.</p>

DOI10.1038/s41467-020-17837-7
Alternate JournalNat Commun
PubMed ID32792484
PubMed Central IDPMC7427095
Grant ListDP2 EB024247 / EB / NIBIB NIH HHS / United States
F32 GM128304 / GM / NIGMS NIH HHS / United States