Development of hybrid scaffolds with natural extracellular matrix deposited within synthetic polymeric fibers.

TitleDevelopment of hybrid scaffolds with natural extracellular matrix deposited within synthetic polymeric fibers.
Publication TypeJournal Article
Year of Publication2017
AuthorsGoyal, R, Vega, ME, Pastino, AK, Singh, S, Guvendiren, M, Kohn, J, N Murthy, S, Schwarzbauer, JE
JournalJ Biomed Mater Res A
Volume105
Issue8
Pagination2162-2170
Date Published2017 Aug
ISSN1552-4965
KeywordsAnimals, Biocompatible Materials, Biopolymers, Cell Adhesion, Cell Line, Cell Movement, Extracellular Matrix, Humans, Mice, NIH 3T3 Cells, Polyethylene Glycols, Porosity, Tissue Engineering, Tissue Scaffolds, Tyrosine
Abstract

<p>A major challenge of tissue engineering is to generate materials that combine bioactivity with stability in a form that captures the robust nature of native tissues. Here we describe a procedure to fabricate a novel hybrid extracellular matrix (ECM)-synthetic scaffold biomaterial by cell-mediated deposition of ECM within an electrospun fiber mat. Synthetic polymer fiber mats were fabricated using poly(desamino tyrosyl-tyrosine carbonate) (PDTEC) co-spun with poly(ethylene glycol) (PEG) used as a sacrificial polymer. PEG removal increased the overall mat porosity and produced a mat with a layered structure that could be peeled into separate sheets of about 50 μm in thickness. Individual layers had pore sizes and wettability that facilitated cell infiltration over the depth of the scaffold. Confocal microscopy showed the formation of a highly interpenetrated network of cells, fibronectin fibrils, and synthetic fibers mimicking a complex ECM as observed within tissues. Decellularization did not perturb the structure of the matrix or the fiber mat. The resulting hybrid ECM-scaffold promoted cell adhesion and spreading and stimulated new ECM assembly by stem cells and tumor cells. These results identify a new technique for fabricating highly porous synthetic fibrous scaffolds and an approach to supplement them with natural biomimetic cues. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2162-2170, 2017.</p>

DOI10.1002/jbm.a.36078
Alternate JournalJ Biomed Mater Res A
PubMed ID28371271
PubMed Central IDPMC5493328
Grant ListT32 EB005583 / EB / NIBIB NIH HHS / United States
P41 EB001046 / EB / NIBIB NIH HHS / United States
T32 GM007388 / GM / NIGMS NIH HHS / United States
R01 CA160611 / CA / NCI NIH HHS / United States
F32 DK109622 / DK / NIDDK NIH HHS / United States