Deubiquitinase USP20 promotes breast cancer metastasis by stabilizing SNAI2. Author Wenyang Li, Minhong Shen, Yi-Zhou Jiang, Ruina Zhang, Hanqiu Zheng, Yong Wei, Zhi-Ming Shao, Yibin Kang Publication Year 2020 Type Journal Article Abstract SNAI2/SLUG, a metastasis-promoting transcription factor, is a labile protein that is degraded through the ubiquitin proteasome degradation system. Here, we conducted comprehensive gain- and loss-of-function screens using a human DUB cDNA library of 65 genes and an siRNA library of 98 genes, and identified USP20 as a deubiquitinase (DUB) that regulates SNAI2 ubiquitination and stability. Further investigation of USP20 demonstrated its function in promoting migration, invasion, and metastasis of breast cancer. USP20 positively correlates with SNAI2 protein level in breast tumor samples, and higher USP20 expression is associated with poor prognosis in ER breast cancer patients. Keywords Humans, Gene Library, Female, RNA, Small Interfering, Cell Movement, Breast Neoplasms, Gene Expression Regulation, Neoplastic, Proteolysis, Ubiquitination, Neoplasm Metastasis, Protein Stability, Snail Family Transcription Factors, Neoplasm Invasiveness, Ubiquitin Thiolesterase Journal Genes Dev Volume 34 Issue 19-20 Pages 1310-1315 Date Published 2020 Oct 01 ISSN Number 1549-5477 DOI 10.1101/gad.339804.120 Alternate Journal Genes Dev PMCID PMC7528704 PMID 32943575 PubMedPubMed CentralGoogle ScholarBibTeXEndNote X3 XML