Deubiquitinase USP20 promotes breast cancer metastasis by stabilizing SNAI2.

TitleDeubiquitinase USP20 promotes breast cancer metastasis by stabilizing SNAI2.
Publication TypeJournal Article
Year of Publication2020
AuthorsLi, W, Shen, M, Jiang, Y-Z, Zhang, R, Zheng, H, Wei, Y, Shao, Z-M, Kang, Y
JournalGenes Dev
Date Published2020 Oct 01
KeywordsBreast Neoplasms, Cell Movement, Female, Gene Expression Regulation, Neoplastic, Gene Library, Humans, Neoplasm Invasiveness, Neoplasm Metastasis, Protein Stability, Proteolysis, RNA, Small Interfering, Snail Family Transcription Factors, Ubiquitin Thiolesterase, Ubiquitination

<p>SNAI2/SLUG, a metastasis-promoting transcription factor, is a labile protein that is degraded through the ubiquitin proteasome degradation system. Here, we conducted comprehensive gain- and loss-of-function screens using a human DUB cDNA library of 65 genes and an siRNA library of 98 genes, and identified USP20 as a deubiquitinase (DUB) that regulates SNAI2 ubiquitination and stability. Further investigation of USP20 demonstrated its function in promoting migration, invasion, and metastasis of breast cancer. USP20 positively correlates with SNAI2 protein level in breast tumor samples, and higher USP20 expression is associated with poor prognosis in ER breast cancer patients.</p>

Alternate JournalGenes Dev
PubMed ID32943575
PubMed Central IDPMC7528704
Grant ListR01 CA141062 / CA / NCI NIH HHS / United States