A de novo enzyme catalyzes a life-sustaining reaction in Escherichia coli.

TitleA de novo enzyme catalyzes a life-sustaining reaction in Escherichia coli.
Publication TypeJournal Article
Year of Publication2018
AuthorsDonnelly, AE, Murphy, GS, Digianantonio, KM, Hecht, MH
JournalNat Chem Biol
Volume14
Issue3
Pagination253-255
Date Published2018 03
ISSN1552-4469
KeywordsCatalysis, Computational Biology, Culture Media, Dimerization, Enterobactin, Escherichia coli, Escherichia coli Proteins, Hydrolysis, Iron, Kinetics, Mutagenesis, Mutation, Phenotype, Protein Folding, Siderophores, Synthetic Biology
Abstract

<p>Producing novel enzymes that are catalytically active in vitro and biologically functional in vivo is a key goal of synthetic biology. Here we describe Syn-F4, the first de novo protein that meets both criteria. Purified Syn-F4 hydrolyzes the siderophore ferric enterobactin, and expression of Syn-F4 allows an inviable strain of Escherichia coli to grow in iron-limited medium. These findings demonstrate that entirely new sequences can provide life-sustaining enzymatic functions in living organisms.</p>

DOI10.1038/nchembio.2550
Alternate JournalNat. Chem. Biol.
PubMed ID29334382