|Title||A de novo enzyme catalyzes a life-sustaining reaction in Escherichia coli.|
|Publication Type||Journal Article|
|Year of Publication||2018|
|Authors||Donnelly, AE, Murphy, GS, Digianantonio, KM, Hecht, MH|
|Journal||Nat Chem Biol|
|Date Published||2018 Jan 15|
Producing novel enzymes that are catalytically active in vitro and biologically functional in vivo is a key goal of synthetic biology. Here we describe Syn-F4, the first de novo protein that meets both criteria. Purified Syn-F4 hydrolyzes the siderophore ferric enterobactin, and expression of Syn-F4 allows an inviable strain of Escherichia coli to grow in iron-limited medium. These findings demonstrate that entirely new sequences can provide life-sustaining enzymatic functions in living organisms.
|Alternate Journal||Nat. Chem. Biol.|