Coupled oscillators coordinate collective germline growth.

TitleCoupled oscillators coordinate collective germline growth.
Publication TypeJournal Article
Year of Publication2021
AuthorsDoherty, CA, Diegmiller, R, Kapasiawala, M, Gavis, ER, Shvartsman, SY
JournalDev Cell
Volume56
Issue6
Pagination860-870.e8
Date Published2021 03 22
ISSN1878-1551
KeywordsAnimals, Cell Communication, Cell Differentiation, Drosophila melanogaster, Drosophila Proteins, Female, Germ Cells, Nuclear Proteins, Oocytes, Oogenesis
Abstract

<p>Developing oocytes need large supplies of macromolecules and organelles. A conserved strategy for accumulating these products is to pool resources of oocyte-associated germline nurse cells. In Drosophila, these cells grow more than 100-fold to boost their biosynthetic capacity. No previously known mechanism explains how nurse cells coordinate growth collectively. Here, we report a cell cycle-regulating mechanism that depends on bidirectional communication between the oocyte and nurse cells, revealing the oocyte as a critical regulator of germline cyst growth. Transcripts encoding the cyclin-dependent kinase inhibitor, Dacapo, are synthesized by the nurse cells and actively localized to the oocyte. Retrograde movement of the oocyte-synthesized Dacapo protein to the nurse cells generates a network of coupled oscillators that controls the cell cycle of the nurse cells to regulate cyst growth. We propose that bidirectional nurse cell-oocyte communication establishes a growth-sensing feedback mechanism that regulates the quantity of maternal resources loaded into the oocyte.</p>

DOI10.1016/j.devcel.2021.02.015
Alternate JournalDev Cell
PubMed ID33689691
PubMed Central IDPMC8265018
Grant ListF31 HD098835 / HD / NICHD NIH HHS / United States
R01 GM134204 / GM / NIGMS NIH HHS / United States
R35 GM126967 / GM / NIGMS NIH HHS / United States
T32 GM007388 / GM / NIGMS NIH HHS / United States