Title | Coupled oscillators coordinate collective germline growth. |
Publication Type | Journal Article |
Year of Publication | 2021 |
Authors | Doherty, CA, Diegmiller, R, Kapasiawala, M, Gavis, ER, Shvartsman, SY |
Journal | Dev Cell |
Volume | 56 |
Issue | 6 |
Pagination | 860-870.e8 |
Date Published | 2021 03 22 |
ISSN | 1878-1551 |
Keywords | Animals, Cell Communication, Cell Differentiation, Drosophila melanogaster, Drosophila Proteins, Female, Germ Cells, Nuclear Proteins, Oocytes, Oogenesis |
Abstract | <p>Developing oocytes need large supplies of macromolecules and organelles. A conserved strategy for accumulating these products is to pool resources of oocyte-associated germline nurse cells. In Drosophila, these cells grow more than 100-fold to boost their biosynthetic capacity. No previously known mechanism explains how nurse cells coordinate growth collectively. Here, we report a cell cycle-regulating mechanism that depends on bidirectional communication between the oocyte and nurse cells, revealing the oocyte as a critical regulator of germline cyst growth. Transcripts encoding the cyclin-dependent kinase inhibitor, Dacapo, are synthesized by the nurse cells and actively localized to the oocyte. Retrograde movement of the oocyte-synthesized Dacapo protein to the nurse cells generates a network of coupled oscillators that controls the cell cycle of the nurse cells to regulate cyst growth. We propose that bidirectional nurse cell-oocyte communication establishes a growth-sensing feedback mechanism that regulates the quantity of maternal resources loaded into the oocyte.</p> |
DOI | 10.1016/j.devcel.2021.02.015 |
Alternate Journal | Dev Cell |
PubMed ID | 33689691 |
PubMed Central ID | PMC8265018 |
Grant List | F31 HD098835 / HD / NICHD NIH HHS / United States R01 GM134204 / GM / NIGMS NIH HHS / United States |