Counting Chromosomes in Individual Bacteria to Quantify Their Impacts on Persistence.

TitleCounting Chromosomes in Individual Bacteria to Quantify Their Impacts on Persistence.
Publication TypeJournal Article
Year of Publication2021
AuthorsMurawski, AM, Rittenbach, K, DeCoste, CJ, Laevsky, G, Brynildsen, MP
JournalMethods Mol Biol
Volume2357
Pagination125-146
Date Published2021/09/30
ISSN1940-6029
Abstract

Persisters are phenotypic variants within bacterial populations that tolerate antibiotic treatments considerably better than the majority of cells. A phenotypic quality that varies within bacterial populations is the chromosome number of individual cells. One, two, four, or more chromosomes per cell have been observed previously, and the impact of genome copy number can range from gene dosage effects to an inability to perform specific DNA repair functions, such as homologous recombination. We hypothesize that chromosome abundance is an underappreciated phenotypic variable that could impact persistence to antibiotics. Here, we describe methodologies to segregate bacterial populations based on chromosome number, assess the purity of those subpopulations, and suggest assays that could be used to quantify the impacts of genome abundance on persistence.

DOI10.1007/978-1-0716-1621-5_9
Alternate JournalMethods Mol Biol
PubMed ID34590256