Counter-rotational cell flows drive morphological and cell fate asymmetries in mammalian hair follicles.

TitleCounter-rotational cell flows drive morphological and cell fate asymmetries in mammalian hair follicles.
Publication TypeJournal Article
Year of Publication2018
AuthorsCetera, M, Leybova, L, Joyce, B, Devenport, D
JournalNat Cell Biol
Volume20
Issue5
Pagination541-552
Date Published2018 May
ISSN1476-4679
Abstract

Organ morphogenesis is a complex process coordinated by cell specification, epithelial-mesenchymal interactions and tissue polarity. A striking example is the pattern of regularly spaced, globally aligned mammalian hair follicles, which emerges through epidermal-dermal signaling and planar polarized morphogenesis. Here, using live-imaging, we discover that developing hair follicles polarize through dramatic cell rearrangements organized in a counter-rotational pattern of cell flows. Upon hair placode induction, Shh signaling specifies a radial pattern of progenitor fates that, together with planar cell polarity, induce counter-rotational rearrangements through myosin and ROCK-dependent polarized neighbour exchanges. Importantly, these cell rearrangements also establish cell fate asymmetry by repositioning radial progenitors along the anterior-posterior axis. These movements concurrently displace associated mesenchymal cells, which then signal asymmetrically to maintain polarized cell fates. Our results demonstrate how spatial patterning and tissue polarity generate an unexpected collective cell behaviour that in turn, establishes both morphological and cell fate asymmetry.

DOI10.1038/s41556-018-0082-7
Alternate JournalNat. Cell Biol.
PubMed ID29662173
PubMed Central IDPMC6065250
Grant ListR01 AR066070 / AR / NIAMS NIH HHS / United States
T32 GM007388 / GM / NIGMS NIH HHS / United States