Confinement size determines the architecture of Ran-induced microtubule networks.

TitleConfinement size determines the architecture of Ran-induced microtubule networks.
Publication TypeJournal Article
Year of Publication2021
AuthorsGai, Y, Cook, B, Setru, S, Stone, HA, Petry, S
JournalSoft Matter
Volume17
Issue24
Pagination5921-5931
Date Published2021 Jun 28
ISSN1744-6848
KeywordsMicrotubule-Associated Proteins, Microtubules, ran GTP-Binding Protein, Spindle Apparatus, Xenopus Proteins
Abstract

<p>The organization of microtubules (MTs) is critical for cells during interphase and mitosis. During mitotic spindle assembly, MTs are made and organized around chromosomes in a process regulated by RanGTP. The role of RanGTP has been explored in Xenopus egg extracts, which are not limited by a cell membrane. Here, we investigated whether cell-sized confinements affect the assembly of RanGTP-induced MT networks in Xenopus egg extracts. We used microfluidics to encapsulate extracts within monodisperse extract-in-oil droplets. Importantly, we find that the architecture of Ran-induced MT networks depends on the droplet diameter and the Ran concentration, and differs from structures formed in bulk extracts. Our results highlight that both MT nucleation and physical confinement play critical roles in determining the spatial organization of the MT cytoskeleton.</p>

DOI10.1039/d1sm00045d
Alternate JournalSoft Matter
PubMed ID34041514
PubMed Central IDPMC8958645
Grant ListDP2 GM123493 / GM / NIGMS NIH HHS / United States