|Title||A Computational Framework for Genome-wide Characterization of the Human Disease Landscape.|
|Publication Type||Journal Article|
|Year of Publication||2019|
|Authors||Lee, Y-S, Krishnan, A, Oughtred, R, Rust, J, Chang, CS, Ryu, J, Kristensen, VN, Dolinski, K, Theesfeld, CL, Troyanskaya, OG|
|Date Published||2019 Feb 27|
A key challenge for the diagnosis and treatment of complex human diseases is identifying their molecular basis. Here, we developed a unified computational framework, URSAHD (Unveiling RNA Sample Annotation for Human Diseases), that leverages machine learning and the hierarchy of anatomical relationships present among diseases to integrate thousands of clinical gene expression profiles and identify molecular characteristics specific to each of the hundreds of complex diseases. URSAHD can distinguish between closely related diseases more accurately than literature-validated genes or traditional differential-expression-based computational approaches and is applicable to any disease, including rare and understudied ones. We demonstrate the utility of URSAHD in classifying related nervous system cancers and experimentally verifying novel neuroblastoma-associated genes identified by URSAHD. We highlight the applications for potential targeted drug-repurposing and for quantitatively assessing the molecular response to clinical therapies. URSAHD is freely available for public use, including the use of underlying models, at ursahd.princeton.edu.
|Alternate Journal||Cell Syst|