Compartmentalized oskar degradation in the germ plasm safeguards germline development.

TitleCompartmentalized oskar degradation in the germ plasm safeguards germline development.
Publication TypeJournal Article
Year of Publication2020
AuthorsEichler, CE, Hakes, AC, Hull, B, Gavis, ER
JournalElife
Volume9
Date Published2020 Jan 07
ISSN2050-084X
Abstract

Partitioning of mRNAs into ribonucleoprotein (RNP) granules supports diverse regulatory programs within the crowded cytoplasm. At least two types of RNP granules populate the germ plasm, a cytoplasmic domain at the posterior of the Drosophila oocyte and embryo. Germ granules deliver mRNAs required for germline development to pole cells, the germ cell progenitors. A second type of RNP granule, here named founder granules, contains oskar mRNA, which encodes the germ plasm organizer. Whereas oskar mRNA is essential for germ plasm assembly during oogenesis, we show that it is toxic to pole cells. Founder granules mediate compartmentalized degradation of oskar during embryogenesis to minimize its inheritance by pole cells. Degradation of oskar in founder granules is temporally and mechanistically distinct from degradation of oskar and other mRNAs during the maternal-to-zygotic transition. Our results show how compartmentalization in RNP granules differentially controls fates of mRNAs localized within the same cytoplasmic domain.

DOI10.7554/eLife.49988
Alternate JournalElife
PubMed ID31909715
PubMed Central IDPMC6986870
Grant ListR35 GM126967 / GM / NIGMS NIH HHS / United States
R01 GM067758 / GM / NIGMS NIH HHS / United States
T32 GM007388 / NH / NIH HHS / United States
T32 GM007388 / GM / NIGMS NIH HHS / United States
R01 GM067758 / NH / NIH HHS / United States
R35 GM126967 / NH / NIH HHS / United States