Coexisting Liquid Phases Underlie Nucleolar Subcompartments.

TitleCoexisting Liquid Phases Underlie Nucleolar Subcompartments.
Publication TypeJournal Article
Year of Publication2016
AuthorsFeric, M, Vaidya, N, Harmon, TS, Mitrea, DM, Zhu, L, Richardson, TM, Kriwacki, RW, Pappu, RV, Brangwynne, CP
JournalCell
Volume165
Issue7
Pagination1686-97
Date Published2016 Jun 16
ISSN0092-8674
Abstract

The nucleolus and other ribonucleoprotein (RNP) bodies are membrane-less organelles that appear to assemble through phase separation of their molecular components. However, many such RNP bodies contain internal subcompartments, and the mechanism of their formation remains unclear. Here, we combine in vivo and in vitro studies, together with computational modeling, to show that subcompartments within the nucleolus represent distinct, coexisting liquid phases. Consistent with their in vivo immiscibility, purified nucleolar proteins phase separate into droplets containing distinct non-coalescing phases that are remarkably similar to nucleoli in vivo. This layered droplet organization is caused by differences in the biophysical properties of the phases-particularly droplet surface tension-which arises from sequence-encoded features of their macromolecular components. These results suggest that phase separation can give rise to multilayered liquids that may facilitate sequential RNA processing reactions in a variety of RNP bodies. PAPERCLIP.

DOI10.1016/j.cell.2016.04.047
Alternate JournalCell
PubMed ID27212236
Grant ListP30 CA021765 / CA / NCI NIH HHS / United States
R01 GM115634 / GM / NIGMS NIH HHS / United States
R01 NS056114 / NS / NINDS NIH HHS / United States