Cloacaenodin, an Antimicrobial Lasso Peptide with Activity against . Author Drew Carson, Monica Patiño, Hader Elashal, Alexis Cartagena, Yi Zhang, Megan Whitley, Larry So, Angelo Kayser-Browne, Ashlee Earl, Roby Bhattacharyya, A James Link Publication Year 2023 Type Journal Article Abstract Using genome mining and heterologous expression, we report the discovery and production of a new antimicrobial lasso peptide from species related to the complex. Using NMR and mass spectrometric analysis, we show that this lasso peptide, named cloacaenodin, employs a threaded lasso fold which imparts proteolytic resistance that its unthreaded counterpart lacks. Cloacaenodin has selective, low micromolar, antimicrobial activity against species related to the complex, including species implicated in nosocomial infections and against clinical isolates of carbapenem-resistant . We further used site-directed mutagenesis to probe the importance of specific residues to the peptide's biosynthesis, stability, and bioactivity. Keywords Anti-Bacterial Agents, Peptides, Antimicrobial Peptides, Enterobacter, Carbapenems Journal ACS Infect Dis Volume 9 Issue 1 Pages 111-121 Date Published 2023 Jan 13 ISSN Number 2373-8227 DOI 10.1021/acsinfecdis.2c00446 Alternate Journal ACS Infect Dis PMCID PMC10038104 PMID 36519726 PubMedPubMed CentralGoogle ScholarBibTeXEndNote X3 XML