Cloacaenodin, an Antimicrobial Lasso Peptide with Activity against .

TitleCloacaenodin, an Antimicrobial Lasso Peptide with Activity against .
Publication TypeJournal Article
Year of Publication2023
AuthorsCarson, DV, Patiño, M, Elashal, HE, Cartagena, AJaramillo, Zhang, Y, Whitley, ME, So, L, Kayser-Browne, AK, Earl, AM, Bhattacharyya, RP, A Link, J
JournalACS Infect Dis
Volume9
Issue1
Pagination111-121
Date Published2023 Jan 13
ISSN2373-8227
KeywordsAnti-Bacterial Agents, Antimicrobial Peptides, Carbapenems, Enterobacter, Peptides
Abstract

<p>Using genome mining and heterologous expression, we report the discovery and production of a new antimicrobial lasso peptide from species related to the complex. Using NMR and mass spectrometric analysis, we show that this lasso peptide, named cloacaenodin, employs a threaded lasso fold which imparts proteolytic resistance that its unthreaded counterpart lacks. Cloacaenodin has selective, low micromolar, antimicrobial activity against species related to the complex, including species implicated in nosocomial infections and against clinical isolates of carbapenem-resistant . We further used site-directed mutagenesis to probe the importance of specific residues to the peptide's biosynthesis, stability, and bioactivity.</p>

DOI10.1021/acsinfecdis.2c00446
Alternate JournalACS Infect Dis
PubMed ID36519726