CLAMP regulates zygotic genome activation in Drosophila embryos.

TitleCLAMP regulates zygotic genome activation in Drosophila embryos.
Publication TypeJournal Article
Year of Publication2021
AuthorsColonnetta, MM, Abrahante, JE, Schedl, P, Gohl, DM, Deshpande, G
JournalGenetics
Volume219
Issue2
Date Published2021 Oct 02
ISSN1943-2631
KeywordsAnimals, Binding Sites, DNA-Binding Proteins, Drosophila melanogaster, Drosophila Proteins, Gene Expression Regulation, Developmental, Nuclear Proteins, Protein Binding, Zygote
Abstract

<p>Embryonic patterning is critically dependent on zygotic genome activation (ZGA). In Drosophila melanogaster embryos, the pioneer factor Zelda directs ZGA, possibly in conjunction with other factors. Here, we have explored the novel involvement of Chromatin-Linked Adapter for MSL Proteins (CLAMP) during ZGA. CLAMP binds thousands of sites genome-wide throughout early embryogenesis. Interestingly, CLAMP relocates to target promoter sequences across the genome when ZGA is initiated. Although there is a considerable overlap between CLAMP and Zelda binding sites, the proteins display distinct temporal dynamics. To assess whether CLAMP occupancy affects gene expression, we analyzed transcriptomes of embryos zygotically compromised for either clamp or zelda and found that transcript levels of many zygotically activated genes are similarly affected. Importantly, compromising either clamp or zelda disrupted the expression of critical segmentation and sex determination genes bound by CLAMP (and Zelda). Furthermore, clamp knockdown embryos recapitulate other phenotypes observed in Zelda-depleted embryos, including nuclear division defects, centrosome aberrations, and a disorganized actomyosin network. Based on these data, we propose that CLAMP acts in concert with Zelda to regulate early zygotic transcription.</p>

DOI10.1093/genetics/iyab107
Alternate JournalGenetics
PubMed ID34849887
PubMed Central IDPMC8633140
Grant ListR21 HD093913 / HD / NICHD NIH HHS / United States
R35 GM126975 / GM / NIGMS NIH HHS / United States