Chromatin landscape signals differentially dictate the activities of mSWI/SNF family complexes. Author Nazar Mashtalir, Hai Dao, Akshay Sankar, Hengyuan Liu, Aaron Corin, John Bagert, Eva Ge, Andrew D'Avino, Martin Filipovski, Brittany Michel, Geoffrey Dann, Tom Muir, Cigall Kadoch Publication Year 2021 Type Journal Article Abstract Mammalian SWI/SNF (mSWI/SNF) adenosine triphosphate-dependent chromatin remodelers modulate genomic architecture and gene expression and are frequently mutated in disease. However, the specific chromatin features that govern their nucleosome binding and remodeling activities remain unknown. We subjected endogenously purified mSWI/SNF complexes and their constituent assembly modules to a diverse library of DNA-barcoded mononucleosomes, performing more than 25,000 binding and remodeling measurements. Here, we define histone modification-, variant-, and mutation-specific effects, alone and in combination, on mSWI/SNF activities and chromatin interactions. Further, we identify the combinatorial contributions of complex module components, reader domains, and nucleosome engagement properties to the localization of complexes to selectively permissive chromatin states. These findings uncover principles that shape the genomic binding and activity of a major chromatin remodeler complex family. Keywords Humans, Transcription Factors, Protein Binding, Mutation, Models, Molecular, Adenosine Triphosphate, Multiprotein Complexes, Adenosine Triphosphatases, Chromosomal Proteins, Non-Histone, Protein Subunits, Histones, Nucleosomes, Chromatin, Chromatin Assembly and Disassembly, Protein Domains, Histone Code Journal Science Volume 373 Issue 6552 Pages 306-315 Date Published 2021 Jul 16 ISSN Number 1095-9203 DOI 10.1126/science.abf8705 Alternate Journal Science PMCID PMC8390793 PMID 34437148 PubMedPubMed CentralGoogle ScholarBibTeXEndNote X3 XML