Title | Chemoenzymatic Semisynthesis of Proteins. |
Publication Type | Journal Article |
Year of Publication | 2020 |
Authors | Thompson, RE, Muir, TW |
Journal | Chem Rev |
Volume | 120 |
Issue | 6 |
Pagination | 3051-3126 |
Date Published | 2020 Mar 25 |
ISSN | 1520-6890 |
Keywords | Chemistry Techniques, Synthetic, Enzymes, Humans, Proteins |
Abstract | <p>Protein semisynthesis-defined herein as the assembly of a protein from a combination of synthetic and recombinant fragments-is a burgeoning field of chemical biology that has impacted many areas in the life sciences. In this review, we provide a comprehensive survey of this area. We begin by discussing the various chemical and enzymatic methods now available for the manufacture of custom proteins containing noncoded elements. This section begins with a discussion of methods that are more chemical in origin and ends with those that employ biocatalysts. We also illustrate the commonalities that exist between these seemingly disparate methods and show how this is allowing for the development of integrated chemoenzymatic methods. This methodology discussion provides the technical foundation for the second part of the review where we cover the great many biological problems that have now been addressed using these tools. Finally, we end the piece with a short discussion on the frontiers of the field and the opportunities available for the future.</p> |
DOI | 10.1021/acs.chemrev.9b00450 |
Alternate Journal | Chem Rev |
PubMed ID | 31774265 |
PubMed Central ID | PMC7101271 |
Grant List | P01 CA196539 / CA / NCI NIH HHS / United States P41 RR000862 / RR / NCRR NIH HHS / United States R37 GM086868 / GM / NIGMS NIH HHS / United States |