Chemoenzymatic Semisynthesis of Proteins.

TitleChemoenzymatic Semisynthesis of Proteins.
Publication TypeJournal Article
Year of Publication2019
AuthorsThompson, RE, Muir, TW
JournalChem Rev
Date Published2019 Nov 27
ISSN1520-6890
Abstract

Protein semisynthesis-defined herein as the assembly of a protein from a combination of synthetic and recombinant fragments-is a burgeoning field of chemical biology that has impacted many areas in the life sciences. In this review, we provide a comprehensive survey of this area. We begin by discussing the various chemical and enzymatic methods now available for the manufacture of custom proteins containing noncoded elements. This section begins with a discussion of methods that are more chemical in origin and ends with those that employ biocatalysts. We also illustrate the commonalities that exist between these seemingly disparate methods and show how this is allowing for the development of integrated chemoenzymatic methods. This methodology discussion provides the technical foundation for the second part of the review where we cover the great many biological problems that have now been addressed using these tools. Finally, we end the piece with a short discussion on the frontiers of the field and the opportunities available for the future.

DOI10.1021/acs.chemrev.9b00450
Alternate JournalChem. Rev.
PubMed ID31774265