Chemoenzymatic Semisynthesis of Proteins.

Publication Year
2020

Type

Journal Article
Abstract

Protein semisynthesis-defined herein as the assembly of a protein from a combination of synthetic and recombinant fragments-is a burgeoning field of chemical biology that has impacted many areas in the life sciences. In this review, we provide a comprehensive survey of this area. We begin by discussing the various chemical and enzymatic methods now available for the manufacture of custom proteins containing noncoded elements. This section begins with a discussion of methods that are more chemical in origin and ends with those that employ biocatalysts. We also illustrate the commonalities that exist between these seemingly disparate methods and show how this is allowing for the development of integrated chemoenzymatic methods. This methodology discussion provides the technical foundation for the second part of the review where we cover the great many biological problems that have now been addressed using these tools. Finally, we end the piece with a short discussion on the frontiers of the field and the opportunities available for the future.

Journal
Chem Rev
Volume
120
Issue
6
Pages
3051-3126
Date Published
2020 Mar 25
ISSN Number
1520-6890
Alternate Journal
Chem Rev
PMCID
PMC7101271
PMID
31774265