The Chemistry and Biology of Bactobolin: A 10-Year Collaboration with Natural Product Chemist Extraordinaire Jon Clardy. Author E Peter Greenberg, Josephine Chandler, Mohammad Seyedsayamdost Publication Year 2020 Type Journal Article Abstract Bactobolin is a hybrid natural product with potent cytotoxic activity. Its production from was reported as part of a collaboration between the Greenberg and Clardy laboratories in 2010. The collaboration sparked a series of studies leading to the discovery of new analogues and associated structure-activity relationships, the identification of the bactobolin biosynthetic gene cluster and assembly of its unusual amino acid building block, the molecular target of and resistance to the antibiotic, and finally an X-ray crystal structure of the ribosome-bactobolin complex. Herein, we review the collaborations that led to our current understanding of the chemistry and biology of bactobolin. Keywords Molecular Structure, Structure-Activity Relationship, Multigene Family, Biological Products, Ribosomes, Benzopyrans, Burkholderia Journal J Nat Prod Volume 83 Issue 3 Pages 738-743 Date Published 2020 Mar 27 ISSN Number 1520-6025 DOI 10.1021/acs.jnatprod.9b01237 Alternate Journal J Nat Prod PMCID PMC8118907 PMID 32105069 PubMedPubMed CentralGoogle ScholarBibTeXEndNote X3 XML