The Chemistry and Biology of Bactobolin: A 10-Year Collaboration with Natural Product Chemist Extraordinaire Jon Clardy.

TitleThe Chemistry and Biology of Bactobolin: A 10-Year Collaboration with Natural Product Chemist Extraordinaire Jon Clardy.
Publication TypeJournal Article
Year of Publication2020
AuthorsE Greenberg, P, Chandler, JR, Seyedsayamdost, MR
JournalJ Nat Prod
Date Published2020 Feb 27
ISSN1520-6025
Abstract

Bactobolin is a hybrid natural product with potent cytotoxic activity. Its production from Burkholderia thailandensis was reported as part of a collaboration between the Greenberg and Clardy laboratories in 2010. The collaboration sparked a series of studies leading to the discovery of new analogues and associated structure-activity relationships, the identification of the bactobolin biosynthetic gene cluster and assembly of its unusual amino acid building block, the molecular target of and resistance to the antibiotic, and finally an X-ray crystal structure of the ribosome-bactobolin complex. Herein, we review the collaborations that led to our current understanding of the chemistry and biology of bactobolin.

DOI10.1021/acs.jnatprod.9b01237
Alternate JournalJ. Nat. Prod.
PubMed ID32105069
Grant ListP20 GM113117 / GM / NIGMS NIH HHS / United States