Chemical biology approaches to study histone interactors.

TitleChemical biology approaches to study histone interactors.
Publication TypeJournal Article
Year of Publication2021
AuthorsBurton, AJ, Hamza, GM, Zhang, AX, Muir, TW
JournalBiochem Soc Trans
Volume49
Issue5
Pagination2431-2441
Date Published2021 11 01
ISSN1470-8752
KeywordsCrystallography, X-Ray, Histones, Mass Spectrometry, Protein Binding, Protein Processing, Post-Translational
Abstract

<p>Protein-protein interactions (PPIs) in the nucleus play key roles in transcriptional regulation and ensure genomic stability. Critical to this are histone-mediated PPI networks, which are further fine-tuned through dynamic post-translational modification. Perturbation to these networks leads to genomic instability and disease, presenting epigenetic proteins as key therapeutic targets. This mini-review will describe progress in mapping the combinatorial histone PTM landscape, and recent chemical biology approaches to map histone interactors. Recent advances in mapping direct interactors of histone PTMs as well as local chromatin interactomes will be highlighted, with a focus on mass-spectrometry based workflows that continue to illuminate histone-mediated PPIs in unprecedented detail.</p>

DOI10.1042/BST20210772
Alternate JournalBiochem Soc Trans
PubMed ID34709376
Grant ListR37 GM086868 / GM / NIGMS NIH HHS / United States