Chaperoning SNARE assembly and disassembly.

TitleChaperoning SNARE assembly and disassembly.
Publication TypeJournal Article
Year of Publication2016
AuthorsBaker, RW, Hughson, FM
JournalNat Rev Mol Cell Biol
Volume17
Issue8
Pagination465-79
Date Published2016 08
ISSN1471-0080
KeywordsAnimals, Humans, Molecular Chaperones, SNARE Proteins
Abstract

<p>Intracellular membrane fusion is mediated in most cases by membrane-bridging complexes of soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs). However, the assembly of such complexes in vitro is inefficient, and their uncatalysed disassembly is undetectably slow. Here, we focus on the cellular machinery that orchestrates assembly and disassembly of SNARE complexes, thereby regulating processes ranging from vesicle trafficking to organelle fusion to neurotransmitter release. Rapid progress is being made on many fronts, including the development of more realistic cell-free reconstitutions, the application of single-molecule biophysics, and the elucidation of X-ray and high-resolution electron microscopy structures of the SNARE assembly and disassembly machineries 'in action'.</p>

DOI10.1038/nrm.2016.65
Alternate JournalNat Rev Mol Cell Biol
PubMed ID27301672
PubMed Central IDPMC5471617
Grant ListR01 GM071574 / GM / NIGMS NIH HHS / United States
T32 GM007388 / GM / NIGMS NIH HHS / United States