Title | Chaperoning SNARE assembly and disassembly. |
Publication Type | Journal Article |
Year of Publication | 2016 |
Authors | Baker, RW, Hughson, FM |
Journal | Nat Rev Mol Cell Biol |
Volume | 17 |
Issue | 8 |
Pagination | 465-79 |
Date Published | 2016 Aug |
ISSN | 1471-0080 |
Keywords | Animals, Humans, Molecular Chaperones, SNARE Proteins |
Abstract | <p>Intracellular membrane fusion is mediated in most cases by membrane-bridging complexes of soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs). However, the assembly of such complexes in vitro is inefficient, and their uncatalysed disassembly is undetectably slow. Here, we focus on the cellular machinery that orchestrates assembly and disassembly of SNARE complexes, thereby regulating processes ranging from vesicle trafficking to organelle fusion to neurotransmitter release. Rapid progress is being made on many fronts, including the development of more realistic cell-free reconstitutions, the application of single-molecule biophysics, and the elucidation of X-ray and high-resolution electron microscopy structures of the SNARE assembly and disassembly machineries 'in action'.</p> |
DOI | 10.1038/nrm.2016.65 |
Alternate Journal | Nat Rev Mol Cell Biol |
PubMed ID | 27301672 |
PubMed Central ID | PMC5471617 |
Grant List | R01 GM071574 / GM / NIGMS NIH HHS / United States T32 GM007388 / GM / NIGMS NIH HHS / United States |