The Chaperone FACT and Histone H2B Ubiquitination Maintain S. pombe Genome Architecture through Genic and Subtelomeric Functions.

TitleThe Chaperone FACT and Histone H2B Ubiquitination Maintain S. pombe Genome Architecture through Genic and Subtelomeric Functions.
Publication TypeJournal Article
Year of Publication2020
AuthorsMurawska, M, Schauer, T, Matsuda, A, Wilson, MD, Pysik, T, Wojcik, F, Muir, TW, Hiraoka, Y, Straub, T, Ladurner, AG
JournalMol Cell
Volume77
Issue3
Pagination501-513.e7
Date Published2020 02 06
ISSN1097-4164
KeywordsChromatin, DNA-Binding Proteins, High Mobility Group Proteins, Histones, Molecular Chaperones, Nucleosomes, Protein Binding, RNA Polymerase II, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Schizosaccharomyces, Schizosaccharomyces pombe Proteins, Transcription Factors, Transcriptional Elongation Factors, Ubiquitination
Abstract

<p>The histone chaperone FACT and histone H2B ubiquitination (H2Bub) facilitate RNA polymerase II (Pol II) passage through chromatin, yet it is not clear how they cooperate mechanistically. We used genomics, genetic, biochemical, and microscopic approaches to dissect their interplay in Schizosaccharomyces pombe. We show that FACT and H2Bub globally repress antisense transcripts near the 5' end of genes and inside gene bodies, respectively. The accumulation of these transcripts is accompanied by changes at genic nucleosomes and Pol II redistribution. H2Bub is required for FACT activity in genic regions. In the H2Bub mutant, FACT binding to chromatin is altered and its association with histones is stabilized, which leads to the reduction of genic nucleosomes. Interestingly, FACT depletion globally restores nucleosomes in the H2Bub mutant. Moreover, in the absence of Pob3, the FACT Spt16 subunit controls the 3' end of genes. Furthermore, FACT maintains nucleosomes in subtelomeric regions, which is crucial for their compaction.</p>

DOI10.1016/j.molcel.2019.11.016
Alternate JournalMol Cell
PubMed ID31837996
PubMed Central IDPMC7007867
Grant List / WT_ / Wellcome Trust / United Kingdom
P01 CA196539 / CA / NCI NIH HHS / United States
R01 GM107047 / GM / NIGMS NIH HHS / United States
R37 GM086868 / GM / NIGMS NIH HHS / United States