Celsr1 adhesive interactions mediate the asymmetric organization of planar polarity complexes.

Publication Year
2021

Type

Journal Article
Abstract

To orchestrate collective polarization across tissues, planar cell polarity (PCP) proteins localize asymmetrically to cell junctions, a conserved feature of PCP that requires the atypical cadherin Celsr1. We report that mouse Celsr1 engages in both - and -interactions, and organizes into dense and highly stable punctate assemblies. We provide evidence suggesting that PCP-mutant variant of Celsr1, Celsr1, selectively impairs lateral -interactions. Although Celsr1 mediates cell adhesion in trans, it displays increased mobility, diminishes junctional enrichment, and fails to engage in homophilic adhesion with the wild-type protein, phenotypes that can be rescued by ectopic -dimerization. Using biochemical and super-resolution microscopy approaches, we show that although Celsr1 physically interacts with PCP proteins Frizzled6 and Vangl2, it fails to organize these proteins into asymmetric junctional complexes. Our results suggest mammalian Celsr1 functions not only as a -adhesive homodimeric bridge, but also as an organizer of intercellular Frizzled6 and Vangl2 asymmetry through lateral, -interactions.

Journal
Elife
Volume
10
Date Published
2021 Feb 02
ISSN Number
2050-084X
Alternate Journal
Elife
PMCID
PMC7857726
PMID
33529151