Cell position fates and collective fountain flow in bacterial biofilms revealed by light-sheet microscopy.

TitleCell position fates and collective fountain flow in bacterial biofilms revealed by light-sheet microscopy.
Publication TypeJournal Article
Year of Publication2020
AuthorsQin, B, Fei, C, Bridges, AA, Mashruwala, AA, Stone, HA, Wingreen, NS, Bassler, BL
JournalScience
Volume369
Issue6499
Pagination71-77
Date Published2020 07 03
ISSN1095-9203
KeywordsBacterial Proteins, Biofilms, Extracellular Matrix Proteins, Microscopy, Motion, Mutation, Single-Cell Analysis, Vibrio cholerae
Abstract

<p>Bacterial biofilms represent a basic form of multicellular organization that confers survival advantages to constituent cells. The sequential stages of cell ordering during biofilm development have been studied in the pathogen and model biofilm-former It is unknown how spatial trajectories of individual cells and the collective motions of many cells drive biofilm expansion. We developed dual-view light-sheet microscopy to investigate the dynamics of biofilm development from a founder cell to a mature three-dimensional community. Tracking of individual cells revealed two distinct fates: one set of biofilm cells expanded ballistically outward, while the other became trapped at the substrate. A collective fountain-like flow transported cells to the biofilm front, bypassing members trapped at the substrate and facilitating lateral biofilm expansion. This collective flow pattern was quantitatively captured by a continuum model of biofilm growth against substrate friction. Coordinated cell movement required the matrix protein RbmA, without which cells expanded erratically. Thus, tracking cell lineages and trajectories in space and time revealed how multicellular structures form from a single founder cell.</p>

DOI10.1126/science.abb8501
Alternate JournalScience
PubMed ID32527924
Grant ListR37 GM065859 / GM / NIGMS NIH HHS / United States
R01 GM082938 / GM / NIGMS NIH HHS / United States
/ HHMI / Howard Hughes Medical Institute / United States