Cell Membranes Resist Flow.

TitleCell Membranes Resist Flow.
Publication TypeJournal Article
Year of Publication2018
AuthorsShi, Z, Graber, ZT, Baumgart, T, Stone, HA, Cohen, AE
JournalCell
Volume175
Issue7
Pagination1769-1779.e13
Date Published2018 12 13
ISSN1097-4172
KeywordsAnimals, Cell Membrane, Cytoskeleton, Dogs, HeLa Cells, Humans, Ion Channels, Madin Darby Canine Kidney Cells, Mice, Models, Biological, NIH 3T3 Cells, Rats, Signal Transduction
Abstract

The fluid-mosaic model posits a liquid-like plasma membrane, which can flow in response to tension gradients. It is widely assumed that membrane flow transmits local changes in membrane tension across the cell in milliseconds, mediating long-range signaling. Here, we show that propagation of membrane tension occurs quickly in cell-attached blebs but is largely suppressed in intact cells. The failure of tension to propagate in cells is explained by a fluid dynamical model that incorporates the flow resistance from cytoskeleton-bound transmembrane proteins. Perturbations to tension propagate diffusively, with a diffusion coefficient Dσ ∼0.024 μm2/s in HeLa cells. In primary endothelial cells, local increases in membrane tension lead only to local activation of mechanosensitive ion channels and to local vesicle fusion. Thus, membrane tension is not a mediator of long-range intracellular signaling, but local variations in tension mediate distinct processes in sub-cellular domains.

DOI10.1016/j.cell.2018.09.054
Alternate JournalCell
PubMed ID30392960
PubMed Central IDPMC6541487
Grant List / / Howard Hughes Medical Institute / United States
R01 GM097552 / GM / NIGMS NIH HHS / United States
U54 CA193417 / CA / NCI NIH HHS / United States