Cell Division Induces and Switches Coherent Angular Motion within Bounded Cellular Collectives.

TitleCell Division Induces and Switches Coherent Angular Motion within Bounded Cellular Collectives.
Publication TypeJournal Article
Year of Publication2017
AuthorsSiedlik, MJ, Manivannan, S, Kevrekidis, IG, Nelson, CM
JournalBiophys J
Volume112
Issue11
Pagination2419-2427
Date Published2017 Jun 06
ISSN1542-0086
Abstract

Collective cell migration underlies many biological processes, including embryonic development, wound healing, and cancer progression. In the embryo, cells have been observed to move collectively in vortices using a mode of collective migration known as coherent angular motion (CAM). To determine how CAM arises within a population and changes over time, here, we study the motion of mammary epithelial cells within engineered monolayers, in which the cells move collectively about a central axis in the tissue. Using quantitative image analysis, we find that CAM is significantly reduced when mitosis is suppressed. Particle-based simulations recreate the observed trends, suggesting that cell divisions drive the robust emergence of CAM and facilitate switches in the direction of collective rotation. Our simulations predict that the location of a dividing cell, rather than the orientation of the division axis, facilitates the onset of this motion. These predictions agree with experimental observations, thereby providing, to our knowledge, new insight into how cell divisions influence CAM within a tissue. Overall, these findings highlight the dynamic nature of CAM and suggest that regulating cell division is crucial for tuning emergent collective migratory behaviors, such as vortical motions observed in vivo.

DOI10.1016/j.bpj.2017.05.001
Alternate JournalBiophys. J.
PubMed ID28591614
PubMed Central IDPMC5474845
Grant ListR21 HL110335 / HL / NHLBI NIH HHS / United States
R01 CA187692 / CA / NCI NIH HHS / United States
R21 HL118532 / HL / NHLBI NIH HHS / United States
R01 GM083997 / GM / NIGMS NIH HHS / United States
R01 HL120142 / HL / NHLBI NIH HHS / United States